TY - JOUR
T1 - Real-time imaging of the resection bed using a handheld probe to reduce incidence of microscopic positive margins in cancer surgery
AU - Erickson-Bhatt, Sarah J.
AU - Nolan, Ryan M.
AU - Shemonski, Nathan D.
AU - Adie, Steven G.
AU - Putney, Jeffrey
AU - Darga, Donald
AU - McCormick, Daniel T.
AU - Cittadine, Andrew J.
AU - Zysk, Adam M.
AU - Marjanovic, Marina
AU - Chaney, Eric J.
AU - Monroy, Guillermo L.
AU - South, Fredrick A.
AU - Cradock, Kimberly A.
AU - Liu, Z. George
AU - Sundaram, Magesh
AU - Ray, Partha S.
AU - Boppart, Stephen A.
N1 - Publisher Copyright:
© 2015 American Association for Cancer Research.
PY - 2015/9/15
Y1 - 2015/9/15
N2 - Wide local excision (WLE) is a common surgical intervention for solid tumors such as those in melanoma, breast, pancreatic, and gastrointestinal cancer. However, adequate margin assessment during WLE remains a significant challenge, resulting in surgical re-interventions to achieve adequate local control. Currently, no label-free imaging method is available for surgeons to examine the resection bed in vivo for microscopic residual cancer. Optical coherence tomography (OCT) enables real-time high-resolution imaging of tissue microstructure. Previous studies have demonstrated that OCT analysis of excised tissue specimens can distinguish between normal and cancerous tissues by identifying the heterogeneous and disorganized microscopic tissue structures indicative of malignancy. In this translational study involving 35 patients, a handheld surgical OCT imaging probe was developed for in vivo use to assess margins both in the resection bed and on excised specimens for themicroscopic presence of cancer. The image results from OCT showed structural differences between normal and cancerous tissue within the resection bed following WLE of the human breast. The ex vivo images were compared with standard postoperative histopathology to yield sensitivity of 91.7% [95% confidence interval (CI), 62.5%-100%] and specificity of 92.1% (95% CI, 78.4%-98%). This study demonstrates in vivo OCT imaging of the resection bed during WLE with the potential for real-time microscopic image-guided surgery. Cancer Res; 75(18); 3706-12.
AB - Wide local excision (WLE) is a common surgical intervention for solid tumors such as those in melanoma, breast, pancreatic, and gastrointestinal cancer. However, adequate margin assessment during WLE remains a significant challenge, resulting in surgical re-interventions to achieve adequate local control. Currently, no label-free imaging method is available for surgeons to examine the resection bed in vivo for microscopic residual cancer. Optical coherence tomography (OCT) enables real-time high-resolution imaging of tissue microstructure. Previous studies have demonstrated that OCT analysis of excised tissue specimens can distinguish between normal and cancerous tissues by identifying the heterogeneous and disorganized microscopic tissue structures indicative of malignancy. In this translational study involving 35 patients, a handheld surgical OCT imaging probe was developed for in vivo use to assess margins both in the resection bed and on excised specimens for themicroscopic presence of cancer. The image results from OCT showed structural differences between normal and cancerous tissue within the resection bed following WLE of the human breast. The ex vivo images were compared with standard postoperative histopathology to yield sensitivity of 91.7% [95% confidence interval (CI), 62.5%-100%] and specificity of 92.1% (95% CI, 78.4%-98%). This study demonstrates in vivo OCT imaging of the resection bed during WLE with the potential for real-time microscopic image-guided surgery. Cancer Res; 75(18); 3706-12.
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U2 - 10.1158/0008-5472.CAN-15-0464
DO - 10.1158/0008-5472.CAN-15-0464
M3 - Article
C2 - 26374464
AN - SCOPUS:84942923365
SN - 0008-5472
VL - 75
SP - 3706
EP - 3712
JO - Cancer Research
JF - Cancer Research
IS - 18
ER -