Reaction of PerR with Molecular Oxygen May Assist H2O2 Sensing in Anaerobes

Ramakrishnan Sethu, Eric Gouré, Luca Signor, Christelle Caux-Thang, Martin Clémancey, Victor Duarte, Jean Marc Latour

Research output: Contribution to journalArticlepeer-review


PerR is the peroxide resistance regulator found in several pathogenic bacteria and governs their resistance to peroxide stress by inducing enzymes that destroy peroxides. However, it has recently been implicated as a key component of the aerotolerance in several facultative or strict anaerobes, including the highly pathogenic Staphylococcus aureus. By combining 18O labeling studies to ESI- and MALDI-TOF MS detection and EMSA experiments, we demonstrate that the active form of PerR reacts with dioxygen, which leads ultimately to disruption of the PerR/DNA complex and is thus physiologically meaningful. Moreover, we show that the presence of O2 assists PerR sensing of H2O2, another feature likely to be important for anaerobic organisms. These results allow one to envisage different scenarios for the response of anaerobes to air exposure.

Original languageEnglish (US)
Pages (from-to)1438-1444
Number of pages7
JournalACS chemical biology
Issue number5
StatePublished - May 20 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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