Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Jefferson Chan, Sheel C. Dodani, Christopher J. Chang

Research output: Contribution to journalReview articlepeer-review


The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.

Original languageEnglish (US)
Pages (from-to)973-984
Number of pages12
JournalNature Chemistry
Issue number12
StatePublished - Dec 2012
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


Dive into the research topics of 'Reaction-based small-molecule fluorescent probes for chemoselective bioimaging'. Together they form a unique fingerprint.

Cite this