Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Jefferson Chan; Sheel C. Dodani; Christopher J. Chang

doi:10.1038/nchem.1500

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Jefferson Chan, Sheel C. Dodani, Christopher J. Chang

Research output: Contribution to journal › Review article › peer-review

Abstract

The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.

Original language	English (US)
Pages (from-to)	973-984
Number of pages	12
Journal	Nature Chemistry
Volume	4
Issue number	12
DOIs	https://doi.org/10.1038/nchem.1500
State	Published - Dec 2012
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1038/nchem.1500

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abstract = "The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.",

author = "Jefferson Chan and Dodani, {Sheel C.} and Chang, {Christopher J.}",

note = "Funding Information: We thank the NIH (GM 79465), the Packard Foundation, Amgen, Astra Zeneca and Novartis for funding our laboratory{\textquoteright}s work on bioimaging. C.J.C. is an Investigator with the Howard Hughes Medical Institute. J.C. thanks the Human Frontiers Science Program for a postdoctoral fellowship and S.C.D. thanks Novartis for a graduate fellowship. We thank L. Lavis for sharing a figure template.",

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Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

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