p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.
ASJC Scopus subject areas
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis