Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

L. Curt Blair, Fred W. Slife, Allan Felsot, Michael Jacob Plewa

Research output: Contribution to journalArticle

Abstract

p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.

Original languageEnglish (US)
Pages (from-to)291-300
Number of pages10
JournalPesticide Biochemistry and Physiology
Volume21
Issue number3
DOIs
StatePublished - Jun 1984

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Daucus carota
Plant Cells
Sulfides
sulfides
carrots
Tobacco
aromatic compounds
Cultured Cells
tobacco
cotton
oxidation
Cell Culture Techniques
Sulfones
cells
cell suspension culture
Suspensions
cell culture
Proteins
proteins

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide. / Blair, L. Curt; Slife, Fred W.; Felsot, Allan; Plewa, Michael Jacob.

In: Pesticide Biochemistry and Physiology, Vol. 21, No. 3, 06.1984, p. 291-300.

Research output: Contribution to journalArticle

Blair, L. Curt ; Slife, Fred W. ; Felsot, Allan ; Plewa, Michael Jacob. / Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide. In: Pesticide Biochemistry and Physiology. 1984 ; Vol. 21, No. 3. pp. 291-300.
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