Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

L. Curt Blair, Fred W. Slife, Allan Felsot, Michael J. Plewa

Research output: Contribution to journalArticle

Abstract

p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.

Original languageEnglish (US)
Pages (from-to)291-300
Number of pages10
JournalPesticide Biochemistry and Physiology
Volume21
Issue number3
DOIs
StatePublished - Jun 1984

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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