Abstract
p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.
Original language | English (US) |
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Pages (from-to) | 291-300 |
Number of pages | 10 |
Journal | Pesticide Biochemistry and Physiology |
Volume | 21 |
Issue number | 3 |
DOIs | |
State | Published - Jun 1984 |
ASJC Scopus subject areas
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis