Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

L. Curt Blair; Fred W. Slife; Allan Felsot; Michael J. Plewa

doi:10.1016/0048-3575(84)90097-X

Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

L. Curt Blair, Fred W. Slife, Allan Felsot, Michael J. Plewa

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO₂), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent K_m for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent V_max was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.

Original language	English (US)
Pages (from-to)	291-300
Number of pages	10
Journal	Pesticide Biochemistry and Physiology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/0048-3575(84)90097-X
State	Published - Jun 1984

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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10.1016/0048-3575(84)90097-X

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title = "Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide",

abstract = "p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.",

author = "Blair, {L. Curt} and Slife, {Fred W.} and Allan Felsot and Plewa, {Michael J.}",

note = "Funding Information: {\textquoteright} Part of a thesis submitted by L.C.B. as partial fulfillment of the Ph.D. degree. Research funded in part by NIEHS Grant ROI ES02384. and Hatch Project I-6-53748. {\textquoteright} To whom reprint requests should be addressed.",

year = "1984",

month = jun,

doi = "10.1016/0048-3575(84)90097-X",

language = "English (US)",

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pages = "291--300",

journal = "Pesticide Biochemistry and Physiology",

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TY - JOUR

T1 - Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

AU - Blair, L. Curt

AU - Slife, Fred W.

AU - Felsot, Allan

AU - Plewa, Michael J.

N1 - Funding Information: ’ Part of a thesis submitted by L.C.B. as partial fulfillment of the Ph.D. degree. Research funded in part by NIEHS Grant ROI ES02384. and Hatch Project I-6-53748. ’ To whom reprint requests should be addressed.

PY - 1984/6

Y1 - 1984/6

N2 - p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.

AB - p-Chlorophenylmethylsulfide (PCPMS) was enzymatically sulfoxidized to p-chlorophenylmethylsulfoxide (PCPMSO) in aerobic cotton, carrot, and tobacco cell suspension cultures. Neither boiled nor freeze-killed cell cultures were competent to sulfoxidize PCPMS to PCPMSO. The sulfone, p-chlorophenylmethylsulfone (PCPMSO2), was not produced in any of the three species. The rates of PCPMS sulfoxidation, were cotton > carrot > tobacco. The apparent Km for carrot cells was 88 μM PCPMS (14 μg/ml), while the apparent Vmax was 7 nmol PCPMSO/mg whole cell protein/hr. These rates of sulfide oxidation are higher than previously reported in intact plants.

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Rates of sulfide oxidation in cotton, carrot, and tobacco cultured plant cells measured with a model aromatic alkyl-sulfide

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