Rapid thermal lysis of cells using silicon-diamond microcantilever heaters

Natalya Privorotskaya, Yi Shao Liu, Jungchul Lee, Hongjun Zeng, John A. Carlisle, Adarsh Radadia, Larry Millet, Rashid Bashir, William P. King

Research output: Contribution to journalArticle

Abstract

This paper presents the design and application of microcantilever heaters for biochemical applications. Thermal lysis of biological cells was demonstrated as a specific example. The microcantilever heaters, fabricated from selectively doped single crystal silicon, provide local resistive heating with highly uniform temperature distribution across the cantilevers. Very importantly, the microcantilever heaters were coated with a layer of 100 nm thick electrically insulating ultrananocrystalline diamond (UNCD) layer used for cell immobilization on the cantilever surface. Fibroblast cells or bacterial cells were immobilized on the UNCD/cantilever surfaces and thermal lysis was demonstrated via optical fluorescence microscopy. Upon electrical heating of the cantilever structures to 93 °C for 30 seconds, fibroblast cell and nuclear membrane were compromised and the cells were lysed. Over 90% of viable bacteria were also lysed after 15 seconds of heating at 93 °C. This work demonstrates the utility of silicon-UNCD heated microcantilevers for rapid cell lysis and forms the basis for other rapid and localized temperature-regulated microbiological experiments in cantilever-based lab on chip applications.

Original languageEnglish (US)
Pages (from-to)1135-1141
Number of pages7
JournalLab on a chip
Volume10
Issue number9
DOIs
StatePublished - 2010

Fingerprint

Diamond
Silicon
Diamonds
Hot Temperature
Fibroblasts
Heating
Cell immobilization
Cells
Fluorescence microscopy
Optical microscopy
Bacteria
Temperature distribution
Immobilized Cells
Temperature
Single crystals
Membranes
Nuclear Envelope
Fluorescence Microscopy
Immobilization
Cell Membrane

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Privorotskaya, N., Liu, Y. S., Lee, J., Zeng, H., Carlisle, J. A., Radadia, A., ... King, W. P. (2010). Rapid thermal lysis of cells using silicon-diamond microcantilever heaters. Lab on a chip, 10(9), 1135-1141. https://doi.org/10.1039/b923791g

Rapid thermal lysis of cells using silicon-diamond microcantilever heaters. / Privorotskaya, Natalya; Liu, Yi Shao; Lee, Jungchul; Zeng, Hongjun; Carlisle, John A.; Radadia, Adarsh; Millet, Larry; Bashir, Rashid; King, William P.

In: Lab on a chip, Vol. 10, No. 9, 2010, p. 1135-1141.

Research output: Contribution to journalArticle

Privorotskaya, N, Liu, YS, Lee, J, Zeng, H, Carlisle, JA, Radadia, A, Millet, L, Bashir, R & King, WP 2010, 'Rapid thermal lysis of cells using silicon-diamond microcantilever heaters', Lab on a chip, vol. 10, no. 9, pp. 1135-1141. https://doi.org/10.1039/b923791g
Privorotskaya N, Liu YS, Lee J, Zeng H, Carlisle JA, Radadia A et al. Rapid thermal lysis of cells using silicon-diamond microcantilever heaters. Lab on a chip. 2010;10(9):1135-1141. https://doi.org/10.1039/b923791g
Privorotskaya, Natalya ; Liu, Yi Shao ; Lee, Jungchul ; Zeng, Hongjun ; Carlisle, John A. ; Radadia, Adarsh ; Millet, Larry ; Bashir, Rashid ; King, William P. / Rapid thermal lysis of cells using silicon-diamond microcantilever heaters. In: Lab on a chip. 2010 ; Vol. 10, No. 9. pp. 1135-1141.
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