TY - JOUR
T1 - Rapid Specific and Label-Free Detection of Porcine Rotavirus Using Photonic Crystal Biosensors
AU - Pineda, Maria Fernanda
AU - Chan, Leo Li Ying
AU - Choi, Charles J.
AU - Cunningham, Brian T.
AU - Kuhlenschmidt, Theresa
AU - Kuhlenschmidt, Mark
N1 - Funding Information:
Manuscript received June 12, 2008; revised August 19, 2008; accepted September 06, 2008. Current version published March 04, 2009. The work of M. F. Pineda and L. L. Chan was contributed equally. This work was supported in part by a grant to MSK from the USDA CSREES NRI Water and Watersheds Program 2006-35102-17344. The associate editor coordinating the review of this paper and approving it for publication was Dr. M. Abedin.
PY - 2009/4
Y1 - 2009/4
N2 - A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.
AB - A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.
KW - Ground water monitoring
KW - label-free detection
KW - photonic crystal biosensor
KW - rotavirus
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U2 - 10.1109/JSEN.2009.2014427
DO - 10.1109/JSEN.2009.2014427
M3 - Article
AN - SCOPUS:85008056677
SN - 1530-437X
VL - 9
SP - 470
EP - 477
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 4
ER -