Rapid, sensitive detection of intact SARS-CoV-2 using DNA nets and a smartphone-linked fluorimeter

Hankeun Lee, Weijing Wang, Neha Chauhan, Yanyu Xiong, Nicholas Magazine, Owen Valdescruz, Dong Yeun Kim, Tianjie Qiu, Weishan Huang, Xing Wang, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a single-step, room-temperature, 5-10 minute SARS-CoV-2 saliva self-monitoring method that overcomes the limitations of existing approaches through the use of fluorophore-releasing Designer DNA Nanostructures (DDNs) that bind with the multivalent pattern of spike proteins on the exterior intact virions and an inexpensive smartphone-linked, pocket-size fluorimeter, called a “V-Pod” for its resemblance to an Apple AirPod™ headphone case. We characterize the V-Pod fluorimeter performance and the DDN-based assay to demonstrate a clinically relevant detection limit of 104 virus particles/mL for pseudo-typed WT SARS-CoV-2 and 105 virus particles/mL for real pathogenic variants, including Delta, Omicron, and D614g.

Original languageEnglish (US)
Title of host publicationOptical Diagnostics and Sensing XXIII
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
PublisherSPIE
ISBN (Electronic)9781510658790
DOIs
StatePublished - 2023
EventOptical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics 2023 - San Francisco, United States
Duration: Jan 30 2023Jan 31 2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12387
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics 2023
Country/TerritoryUnited States
CitySan Francisco
Period1/30/231/31/23

Keywords

  • Biosensor
  • COVID-19 testing
  • Designer DNA nanostructure
  • Point-of-care test
  • Portable Fluorometer
  • Smartphone

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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