Rapid prototyping of microbial cell factories via genome-scale engineering

Tong Si, Han Xiao, Huimin Zhao

Research output: Contribution to journalReview articlepeer-review


Advances in reading, writing and editing genetic materials have greatly expanded our ability to reprogram biological systems at the resolution of a single nucleotide and on the scale of a whole genome. Such capacity has greatly accelerated the cycles of design, build and test to engineer microbes for efficient synthesis of fuels, chemicals and drugs. In this review, we summarize the emerging technologies that have been applied, or are potentially useful for genome-scale engineering in microbial systems. We will focus on the development of high-throughput methodologies, which may accelerate the prototyping of microbial cell factories.

Original languageEnglish (US)
Pages (from-to)1420-1432
Number of pages13
JournalBiotechnology Advances
Issue number7
StatePublished - Nov 15 2015


  • Genome synthesis
  • Genome-scale engineering
  • High-throughput technology
  • Homologous recombination
  • Microbial cell factory
  • Transcriptome engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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