Rapid measurement of total antioxidant capacity in plants

Kelly M. Gillespie; June M. Chae; Elizabeth A. Ainsworth

doi:10.1038/nprot.2007.100

Rapid measurement of total antioxidant capacity in plants

Kelly M. Gillespie, June M. Chae, Elizabeth A. Ainsworth

Research output: Contribution to journal › Article › peer-review

Abstract

There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.

Original language	English (US)
Pages (from-to)	867-870
Number of pages	4
Journal	Nature Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1038/nprot.2007.100
State	Published - Apr 2007

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Online availability

10.1038/nprot.2007.100

Library availability

Discover UIUC Full Text

Cite this

@article{00f6cb622bd84c0ca17c0c48d1eaedc4,

title = "Rapid measurement of total antioxidant capacity in plants",

abstract = "There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.",

author = "Gillespie, {Kelly M.} and Chae, {June M.} and Ainsworth, {Elizabeth A.}",

note = "Funding Information: ACKNOWLEDGMENTS This research was supported by the Office of Science (BER), U.S. Department of Energy, Grant no. DE-FG02-04ER63849.",

year = "2007",

month = apr,

doi = "10.1038/nprot.2007.100",

language = "English (US)",

volume = "2",

pages = "867--870",

journal = "Nature Protocols",

issn = "1754-2189",

publisher = "Nature Publishing Group",

number = "4",

}

TY - JOUR

T1 - Rapid measurement of total antioxidant capacity in plants

AU - Gillespie, Kelly M.

AU - Chae, June M.

AU - Ainsworth, Elizabeth A.

N1 - Funding Information: ACKNOWLEDGMENTS This research was supported by the Office of Science (BER), U.S. Department of Energy, Grant no. DE-FG02-04ER63849.

PY - 2007/4

Y1 - 2007/4

N2 - There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.

AB - There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.

UR - http://www.scopus.com/inward/record.url?scp=34248592696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248592696&partnerID=8YFLogxK

U2 - 10.1038/nprot.2007.100

DO - 10.1038/nprot.2007.100

M3 - Article

C2 - 17446887

AN - SCOPUS:34248592696

SN - 1754-2189

VL - 2

SP - 867

EP - 870

JO - Nature Protocols

JF - Nature Protocols

IS - 4

ER -

Rapid measurement of total antioxidant capacity in plants

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this