Abstract
There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.
Original language | English (US) |
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Pages (from-to) | 867-870 |
Number of pages | 4 |
Journal | Nature Protocols |
Volume | 2 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology