Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: Toward in vivo implementation

Youbo Zhao, Guillermo L. Monroy, Sixian You, Ryan L. Shelton, Ryan M. Nolan, Haohua Tu, Eric J. Chaney, Stephen A. Boppart

Research output: Contribution to journalArticlepeer-review

Abstract

We investigate and demonstrate the feasibility of using a combined Raman scattering (RS) spectroscopy and low-coherence interferometry (LCI) probe to differentiate microbial pathogens and improve our diagnostic ability of ear infections [otitis media (OM)]. While the RS probe provides noninvasive molecular information to identify and differentiate infectious microorganisms, the LCI probe helps to identify depth-resolved structural information as well as to guide and monitor positioning of the Raman spectroscopy beam for relatively longer signal acquisition times. A series of phantom studies, including the use of human middle ear effusion samples, were performed to mimic the conditions of in vivo investigations. These were also conducted to validate the feasibility of using this combined RS/LCI probe for point-of-care diagnosis of the infectious pathogen(s) in OM patients. This work establishes important parameters for future in vivo investigations of fast and accurate determination and diagnosis of infectious microorganisms in OM patients, potentially improving the efficacy and outcome of OM treatments, and importantly reducing the misuse of antibiotics in the presence of viral infections.

Original languageEnglish (US)
Article number107005
JournalJournal of biomedical optics
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2016

Keywords

  • Raman spectroscopy
  • biomedical optics instrumentation
  • optical coherence tomography
  • optical diagnostics for medicine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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