TY - JOUR
T1 - Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe
T2 - Toward in vivo implementation
AU - Zhao, Youbo
AU - Monroy, Guillermo L.
AU - You, Sixian
AU - Shelton, Ryan L.
AU - Nolan, Ryan M.
AU - Tu, Haohua
AU - Chaney, Eric J.
AU - Boppart, Stephen A.
N1 - Funding Information:
This research was supported in part by grants from the Center for Integration of Medicine and Innovative Technology (S.A.B. and R.L.S.), the National Institutes of Health (R01EB013723, S.A.B.), and the National Science Foundation (CBET 14-45111, S.A.B.).
Publisher Copyright:
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2016/10/1
Y1 - 2016/10/1
N2 - We investigate and demonstrate the feasibility of using a combined Raman scattering (RS) spectroscopy and low-coherence interferometry (LCI) probe to differentiate microbial pathogens and improve our diagnostic ability of ear infections [otitis media (OM)]. While the RS probe provides noninvasive molecular information to identify and differentiate infectious microorganisms, the LCI probe helps to identify depth-resolved structural information as well as to guide and monitor positioning of the Raman spectroscopy beam for relatively longer signal acquisition times. A series of phantom studies, including the use of human middle ear effusion samples, were performed to mimic the conditions of in vivo investigations. These were also conducted to validate the feasibility of using this combined RS/LCI probe for point-of-care diagnosis of the infectious pathogen(s) in OM patients. This work establishes important parameters for future in vivo investigations of fast and accurate determination and diagnosis of infectious microorganisms in OM patients, potentially improving the efficacy and outcome of OM treatments, and importantly reducing the misuse of antibiotics in the presence of viral infections.
AB - We investigate and demonstrate the feasibility of using a combined Raman scattering (RS) spectroscopy and low-coherence interferometry (LCI) probe to differentiate microbial pathogens and improve our diagnostic ability of ear infections [otitis media (OM)]. While the RS probe provides noninvasive molecular information to identify and differentiate infectious microorganisms, the LCI probe helps to identify depth-resolved structural information as well as to guide and monitor positioning of the Raman spectroscopy beam for relatively longer signal acquisition times. A series of phantom studies, including the use of human middle ear effusion samples, were performed to mimic the conditions of in vivo investigations. These were also conducted to validate the feasibility of using this combined RS/LCI probe for point-of-care diagnosis of the infectious pathogen(s) in OM patients. This work establishes important parameters for future in vivo investigations of fast and accurate determination and diagnosis of infectious microorganisms in OM patients, potentially improving the efficacy and outcome of OM treatments, and importantly reducing the misuse of antibiotics in the presence of viral infections.
KW - Raman spectroscopy
KW - biomedical optics instrumentation
KW - optical coherence tomography
KW - optical diagnostics for medicine
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U2 - 10.1117/1.JBO.21.10.107005
DO - 10.1117/1.JBO.21.10.107005
M3 - Article
C2 - 27802456
AN - SCOPUS:84994481717
VL - 21
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 10
M1 - 107005
ER -