Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

Zengyi Shao, Yunzi Luo, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

Original languageEnglish (US)
Pages (from-to)1056-1059
Number of pages4
JournalMolecular BioSystems
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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