TY - JOUR
T1 - Random sequential adsorption of human adenovirus 2 onto polyvinylidene fluoride surface influenced by extracellular polymeric substances
AU - Lu, Ruiqing
AU - Li, Qi
AU - Nguyen, Thanh H.
N1 - This work is supported by the National Science Foundation in CBET Grant 1236672 . The authors want to especially acknowledge Bernardo Vazquez Bravo and Aimee Gall for teaching us the techniques to work with human adenovirus. We also want to acknowledge Dean Olson for his help with NMR.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - Virus removal by membrane bioreactors depends on virus-membrane and virus-foulant interactions. The adsorption of human adenovirus 2 (HAdV-2) on polyvinylidene fluoride (PVDF) membrane and a major membrane foulant, extracellular polymeric substances (EPS), were measured in a quartz crystal microbalance. In 3-100mM CaCl2 solutions, irreversible adsorption of HAdV-2 was observed on both pristine and EPS-fouled PVDF surfaces. The HAdV-2 adsorption kinetics was successfully fitted with the random sequential adsorption (RSA) model. The applicability of the RSA model for HAdV-2 adsorption is confirmed by comparing the two fitting parameters, adsorption rate constant ka and area occupied by each adsorbed HAdV-2 particle a, with experimentally measured parameters. A linear correlation between the fitting parameter ka and the measured attachment efficiency was found, suggesting that the RSA model correctly describes the interaction forces dominating the HAdV-2 adsorption. By comparing the fitting parameter dads with the hydrodynamic diameter of HAdV-2, we conclude that virus-virus and virus-surface interactions determine the area occupied by each adsorbed HAdV-2 particle, and thus influence the adsorption capacity. These results provide insights into virus retention and will benefit improving virus removal in membrane filtration.
AB - Virus removal by membrane bioreactors depends on virus-membrane and virus-foulant interactions. The adsorption of human adenovirus 2 (HAdV-2) on polyvinylidene fluoride (PVDF) membrane and a major membrane foulant, extracellular polymeric substances (EPS), were measured in a quartz crystal microbalance. In 3-100mM CaCl2 solutions, irreversible adsorption of HAdV-2 was observed on both pristine and EPS-fouled PVDF surfaces. The HAdV-2 adsorption kinetics was successfully fitted with the random sequential adsorption (RSA) model. The applicability of the RSA model for HAdV-2 adsorption is confirmed by comparing the two fitting parameters, adsorption rate constant ka and area occupied by each adsorbed HAdV-2 particle a, with experimentally measured parameters. A linear correlation between the fitting parameter ka and the measured attachment efficiency was found, suggesting that the RSA model correctly describes the interaction forces dominating the HAdV-2 adsorption. By comparing the fitting parameter dads with the hydrodynamic diameter of HAdV-2, we conclude that virus-virus and virus-surface interactions determine the area occupied by each adsorbed HAdV-2 particle, and thus influence the adsorption capacity. These results provide insights into virus retention and will benefit improving virus removal in membrane filtration.
KW - Adenovirus
KW - Extracellular polymeric substances
KW - Membrane filtration
KW - Random sequential adsorption
UR - https://www.scopus.com/pages/publications/84951167978
UR - https://www.scopus.com/pages/publications/84951167978#tab=citedBy
U2 - 10.1016/j.jcis.2015.12.017
DO - 10.1016/j.jcis.2015.12.017
M3 - Article
C2 - 26720514
AN - SCOPUS:84951167978
SN - 0021-9797
VL - 466
SP - 120
EP - 127
JO - Journal of Colloid And Interface Science
JF - Journal of Colloid And Interface Science
ER -