Raman spectroscopy characterization of diabetes effects on human vitreous in diabetic retinopathy

J. Sebag, S. Nie, K. Reiser, N. T. Yu

Research output: Contribution to journalConference articlepeer-review


Nonenzymatic glycation alters collagen throughout the body, resulting in the histopathology that underlies diabetic disease in several organs (1-3). In the eye such changes in vitreous collagen could contribute to the progression of proliferative diabetic retinopathy by inducing vitreous degeneration (4). Previous studies (5,6) have demonstrated early glycation and advanced glycation endproducts in the vitreous of humans with proliferative diabetic retinopathy. Subsequent studies (7) have found both nonenzymatic glycation and enzyme-mediated crosslinks of collagen. In this study, Near Infrared Fourier-Transform Raman Spectroscopy was performed on vitreous obtained at surgery from diabetic patients and from non-diabetic control subjects (8). The findings were compared to measurements obtained in untreated and glycated (in vitro) rat-tail tendon collagen, as well as demineralized chick bone, rich in crosslinks. The results demonstrated substantial changes in diabetic vitreous collagen not resulting from enzyme-mediated crosslinking, but most likely advanced nonenzymatic glycation. This approach appears to be useful as a means of characterizing the molecular changes induced by diabetes. Furthermore, this technique could be developed as a way of quantifying these changes in vivo in several tissues, so as to gauge the severity of disease and monitor the response to therapy.

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Jun 24 1993
Externally publishedYes
EventOphthalmic Technologies III 1993 - Los Angeles, United States
Duration: Jan 17 1993Jan 22 1993

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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