Raman multiplexers for alternative gene splicing

Lan Sun, Chenxu Yu, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). Afour-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Δ(11q) (the last 3309 nt deleted from exon 11), Δ(9, 10) (exon 9 and 10 deleted), Δ(5) (exon 5 deleted), and Δ(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases.

Original languageEnglish (US)
Pages (from-to)3342-3349
Number of pages8
JournalAnalytical chemistry
Volume80
Issue number9
DOIs
StatePublished - May 1 2008
Externally publishedYes

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Exons
Genes
Labels
Raman scattering
DNA sequences
Alternative Splicing

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Raman multiplexers for alternative gene splicing. / Sun, Lan; Yu, Chenxu; Irudayaraj, Joseph.

In: Analytical chemistry, Vol. 80, No. 9, 01.05.2008, p. 3342-3349.

Research output: Contribution to journalArticle

Sun, Lan ; Yu, Chenxu ; Irudayaraj, Joseph. / Raman multiplexers for alternative gene splicing. In: Analytical chemistry. 2008 ; Vol. 80, No. 9. pp. 3342-3349.
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