Raman chemical imaging of chromate reduction sites in a single bacterium using intracellularly grown gold nanoislands

Sandeep P. Ravindranath, Kristene L. Henne, Dorothea K. Thompson, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Imaging live molecular events within micro-organisms at single-cell resolution would deliver valuable mechanistic information much needed in understanding key biological processes. We present a surface-enhanced Raman (SERS) chemical imaging strategy as a first step toward exploring the intracellular bioreduction pockets of toxic chromate in Shewanella. In order to achieve this, we take advantage of an innate reductive mechanism in bacteria of reducing gold ions into intracellular gold nanoislands, which provide the necessary enhancement for SERS imaging. We show that SERS has the sensitivity and selectivity not only to identify but also to differentiate between the two stable valence forms of chromate in cells. The imaging platform was used to understand intracellular metal reduction activities in a ubiquitous metal-reducing organism, Shewanella oneidensis MR-1, by mapping chromate reduction.

Original languageEnglish (US)
Pages (from-to)4729-4736
Number of pages8
JournalACS Nano
Volume5
Issue number6
DOIs
StatePublished - Jun 28 2011

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Chromates
chromates
Gold
bacteria
Bacteria
gold
organisms
Imaging techniques
Metals
resolution cell
Molecular imaging
Poisons
metals
platforms
selectivity
Ions
valence
augmentation
sensitivity
cells

Keywords

  • Shewanella oneidensis MR-1
  • bioremediation
  • hexavalent chromate
  • single-cell Raman imaging
  • surface-enhanced Raman spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Raman chemical imaging of chromate reduction sites in a single bacterium using intracellularly grown gold nanoislands. / Ravindranath, Sandeep P.; Henne, Kristene L.; Thompson, Dorothea K.; Irudayaraj, Joseph.

In: ACS Nano, Vol. 5, No. 6, 28.06.2011, p. 4729-4736.

Research output: Contribution to journalArticle

Ravindranath, Sandeep P. ; Henne, Kristene L. ; Thompson, Dorothea K. ; Irudayaraj, Joseph. / Raman chemical imaging of chromate reduction sites in a single bacterium using intracellularly grown gold nanoislands. In: ACS Nano. 2011 ; Vol. 5, No. 6. pp. 4729-4736.
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