TY - JOUR
T1 - Radiochemical assay of juvenile hormone biosynthesis rate in ants
AU - Brent, Colin
AU - Dolezal, Adam
PY - 2009
Y1 - 2009
N2 - Juvenile hormone (JH) is an important insect hormone known to have many effects on development, reproduction, and behavior in both solitary and social insects. This protocol describes how to quantify in vitro biosynthesis rates from excised corpora allata (CA), the glands responsible for JH production. Excised glands are incubated with radiolabeled methionine, resulting in the production of radiolabeled JH III, which can then be quantified. This protocol is most useful when quantification of the activity of the glands, rather than circulating JH levels, is desired. Its difficulties lie primarily in the ability to remove and handle the glands correctly.
AB - Juvenile hormone (JH) is an important insect hormone known to have many effects on development, reproduction, and behavior in both solitary and social insects. This protocol describes how to quantify in vitro biosynthesis rates from excised corpora allata (CA), the glands responsible for JH production. Excised glands are incubated with radiolabeled methionine, resulting in the production of radiolabeled JH III, which can then be quantified. This protocol is most useful when quantification of the activity of the glands, rather than circulating JH levels, is desired. Its difficulties lie primarily in the ability to remove and handle the glands correctly.
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U2 - 10.1101/pdb.prot5248
DO - 10.1101/pdb.prot5248
M3 - Article
C2 - 20147213
AN - SCOPUS:73349115222
SN - 1559-6095
VL - 4
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 7
ER -