Quantum dot/antibody conjugates for in vivo cytometric imaging in mice

Hee Sun Han, Elisabeth Niemeyer, Yuhui Huang, Walid S. Kamoun, John D. Martin, Jayeeta Bhaumik, Yunching Chen, Sylvie Roberge, Jian Cui, Margaret R. Martin, Dai Fukumura, Rakesh K. Jainb, Moungi G. Bawendi, Dan G. Duda

Research output: Contribution to journalArticlepeer-review

Abstract

Multiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugates must be stable and maintain a high quantum yield in the in vivo environments. Here, we show that this can be achieved using compact (∼15 nm in hydrodynamic diameter) and biocompatible quantum dot (QD) -Ab conjugates. We developed these conjugates by coupling whole mAbs to QDs coated with norbornene-displaying polyimidazole ligands using tetrazine -norbornene cycloaddition. Our QD immunoconstructs were used for in vivo single-cell labeling in bone marrow. The intravital imaging studies using a chronic calvarial bone window showed that our QD-Ab conjugates diffuse into the entire bone marrow and efficiently label single cells belonging to rare populations of hematopoietic stem and progenitor cells (Sca1+c-Kit+ cells). This in vivo cytometric technique may be useful in a wide range of structural and functional imaging to study the interactions between cells and between a cell and its environment in intact and diseased tissues. quantum dots in vivo imaging single cell imaging in vivo cytometry multiphoton microscopy.

Original languageEnglish (US)
Pages (from-to)1350-1355
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number5
DOIs
StatePublished - Feb 3 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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