Quantitative phase imaging of arthropods

Shamira Sridharan, Aron Katz, Felipe Soto-Adames, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review


Classification of arthropods is performed by characterization of fine features such as setae and cuticles. An unstained whole arthropod specimen mounted on a slide can be preserved for many decades, but is difficult to study since current methods require sample manipulation or tedious image processing. Spatial light interference microscopy (SLIM) is a quantitative phase imaging (QPI) technique that is an add-on module to a commercial phase contrast microscope. We use SLIM to image a whole organism springtail Ceratophysella denticulata mounted on a slide. This is the first time, to our knowledge, that an entire organism has been imaged using QPI. We also demonstrate the ability of SLIM to image fine structures in addition to providing quantitative data that cannot be obtained by traditional bright field microscopy.

Original languageEnglish (US)
Article number111212
JournalJournal of biomedical optics
Issue number11
StatePublished - Nov 1 2015


  • arthropod
  • biomedical optics
  • classification
  • microscopy
  • quantitative phase imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


Dive into the research topics of 'Quantitative phase imaging of arthropods'. Together they form a unique fingerprint.

Cite this