Quantitative neuroproteomics of the synapse.

Dinah Lee Ramos-Ortolaza, Ittai Bushlin, Noura Abul-Husn, Suresh P. Annangudi, Jonathan V Sweedler, Lakshmi A. Devi

Research output: Contribution to journalArticle

Abstract

An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

Original languageEnglish (US)
Pages (from-to)227-246
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume615
StatePublished - 2010
Externally publishedYes

Fingerprint

Proteomics
Synapses
Neurodegenerative Diseases
Proteins
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ramos-Ortolaza, D. L., Bushlin, I., Abul-Husn, N., Annangudi, S. P., Sweedler, J. V., & Devi, L. A. (2010). Quantitative neuroproteomics of the synapse. Methods in molecular biology (Clifton, N.J.), 615, 227-246.

Quantitative neuroproteomics of the synapse. / Ramos-Ortolaza, Dinah Lee; Bushlin, Ittai; Abul-Husn, Noura; Annangudi, Suresh P.; Sweedler, Jonathan V; Devi, Lakshmi A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 615, 2010, p. 227-246.

Research output: Contribution to journalArticle

Ramos-Ortolaza, DL, Bushlin, I, Abul-Husn, N, Annangudi, SP, Sweedler, JV & Devi, LA 2010, 'Quantitative neuroproteomics of the synapse.', Methods in molecular biology (Clifton, N.J.), vol. 615, pp. 227-246.
Ramos-Ortolaza DL, Bushlin I, Abul-Husn N, Annangudi SP, Sweedler JV, Devi LA. Quantitative neuroproteomics of the synapse. Methods in molecular biology (Clifton, N.J.). 2010;615:227-246.
Ramos-Ortolaza, Dinah Lee ; Bushlin, Ittai ; Abul-Husn, Noura ; Annangudi, Suresh P. ; Sweedler, Jonathan V ; Devi, Lakshmi A. / Quantitative neuroproteomics of the synapse. In: Methods in molecular biology (Clifton, N.J.). 2010 ; Vol. 615. pp. 227-246.
@article{3949f989eb134e22a783c653eb23ded7,
title = "Quantitative neuroproteomics of the synapse.",
abstract = "An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.",
author = "Ramos-Ortolaza, {Dinah Lee} and Ittai Bushlin and Noura Abul-Husn and Annangudi, {Suresh P.} and Sweedler, {Jonathan V} and Devi, {Lakshmi A.}",
year = "2010",
language = "English (US)",
volume = "615",
pages = "227--246",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Quantitative neuroproteomics of the synapse.

AU - Ramos-Ortolaza, Dinah Lee

AU - Bushlin, Ittai

AU - Abul-Husn, Noura

AU - Annangudi, Suresh P.

AU - Sweedler, Jonathan V

AU - Devi, Lakshmi A.

PY - 2010

Y1 - 2010

N2 - An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

AB - An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

UR - http://www.scopus.com/inward/record.url?scp=77449123424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449123424&partnerID=8YFLogxK

M3 - Article

C2 - 20013213

AN - SCOPUS:77449123424

VL - 615

SP - 227

EP - 246

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -