Quantitative neuroproteomics of the synapse.

Dinah Lee Ramos-Ortolaza, Ittai Bushlin, Noura Abul-Husn, Suresh P. Annangudi, Jonathan Sweedler, Lakshmi A. Devi

Research output: Contribution to journalArticle

Abstract

An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

Original languageEnglish (US)
Pages (from-to)227-246
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume615
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Quantitative neuroproteomics of the synapse.'. Together they form a unique fingerprint.

  • Cite this

    Ramos-Ortolaza, D. L., Bushlin, I., Abul-Husn, N., Annangudi, S. P., Sweedler, J., & Devi, L. A. (2010). Quantitative neuroproteomics of the synapse. Methods in molecular biology (Clifton, N.J.), 615, 227-246. https://doi.org/10.1007/978-1-60761-535-4_18