Quantitative investigation of compartmentalized dynamics of ErbB2 targeting gold nanorods in live cells by single molecule spectroscopy

Research output: Contribution to journalArticle

Abstract

Understanding the diffusion dynamics and receptor uptake mechanism of nanoparticles in cancer cells is crucial to the rational design of multifunctional nanoprobes for targeting and delivery. In this report, for the first time, we quantify the localization and evaluate the diffusion times of Herceptin-conjugated gold nanorods (H-GNRs) in different cell organelles by fluorescence correlation spectroscopy (FCS) and examine the endocytic diffusion of H-GNRs in live ErbB2 overexpressing SK-BR-3 cells. First, by colocalizing H-GNRs in different cellular organelles depicted by the respective markers, we demonstrate that H-GNRs colocalize with the endosome and lysosome but not with the Golgi apparatus. Our study shows that Herceptin-conjugated GNRs have similar intracellular localization characteristics as Herceptin - ErbB2 complex, with a higher concentration found in the endosome (72 ± 20.6 nM) than lysosome (9.4 ± 4.2 nM) after internalization. The demonstrated approach and findings not only lay the foundations for a quantitative understanding of the fate of nanoparticle-based targeting but also provide new insights into the rational design of nanoparticle delivery systems for effective treatment.

Original languageEnglish (US)
Pages (from-to)4071-4079
Number of pages9
JournalACS Nano
Volume3
Issue number12
DOIs
StatePublished - Dec 22 2009

Fingerprint

Nanorods
Gold
nanorods
Spectroscopy
gold
lysosomes
organelles
Molecules
Nanoparticles
cells
nanoparticles
spectroscopy
molecules
delivery
Nanoprobes
markers
cancer
Fluorescence
Cells
fluorescence

Keywords

  • Compartmentalization
  • Endocytosis
  • ErbB2
  • Fluorescence correlation spectroscopy
  • Herceptin - gold nanorods
  • Quantification

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Quantitative investigation of compartmentalized dynamics of ErbB2 targeting gold nanorods in live cells by single molecule spectroscopy. / Chen, Jiji; Irudayaraj, Joseph.

In: ACS Nano, Vol. 3, No. 12, 22.12.2009, p. 4071-4079.

Research output: Contribution to journalArticle

@article{15681d4608ad4531bf8204a23446025a,
title = "Quantitative investigation of compartmentalized dynamics of ErbB2 targeting gold nanorods in live cells by single molecule spectroscopy",
abstract = "Understanding the diffusion dynamics and receptor uptake mechanism of nanoparticles in cancer cells is crucial to the rational design of multifunctional nanoprobes for targeting and delivery. In this report, for the first time, we quantify the localization and evaluate the diffusion times of Herceptin-conjugated gold nanorods (H-GNRs) in different cell organelles by fluorescence correlation spectroscopy (FCS) and examine the endocytic diffusion of H-GNRs in live ErbB2 overexpressing SK-BR-3 cells. First, by colocalizing H-GNRs in different cellular organelles depicted by the respective markers, we demonstrate that H-GNRs colocalize with the endosome and lysosome but not with the Golgi apparatus. Our study shows that Herceptin-conjugated GNRs have similar intracellular localization characteristics as Herceptin - ErbB2 complex, with a higher concentration found in the endosome (72 ± 20.6 nM) than lysosome (9.4 ± 4.2 nM) after internalization. The demonstrated approach and findings not only lay the foundations for a quantitative understanding of the fate of nanoparticle-based targeting but also provide new insights into the rational design of nanoparticle delivery systems for effective treatment.",
keywords = "Compartmentalization, Endocytosis, ErbB2, Fluorescence correlation spectroscopy, Herceptin - gold nanorods, Quantification",
author = "Jiji Chen and Joseph Irudayaraj",
year = "2009",
month = "12",
day = "22",
doi = "10.1021/nn900743v",
language = "English (US)",
volume = "3",
pages = "4071--4079",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Quantitative investigation of compartmentalized dynamics of ErbB2 targeting gold nanorods in live cells by single molecule spectroscopy

AU - Chen, Jiji

AU - Irudayaraj, Joseph

PY - 2009/12/22

Y1 - 2009/12/22

N2 - Understanding the diffusion dynamics and receptor uptake mechanism of nanoparticles in cancer cells is crucial to the rational design of multifunctional nanoprobes for targeting and delivery. In this report, for the first time, we quantify the localization and evaluate the diffusion times of Herceptin-conjugated gold nanorods (H-GNRs) in different cell organelles by fluorescence correlation spectroscopy (FCS) and examine the endocytic diffusion of H-GNRs in live ErbB2 overexpressing SK-BR-3 cells. First, by colocalizing H-GNRs in different cellular organelles depicted by the respective markers, we demonstrate that H-GNRs colocalize with the endosome and lysosome but not with the Golgi apparatus. Our study shows that Herceptin-conjugated GNRs have similar intracellular localization characteristics as Herceptin - ErbB2 complex, with a higher concentration found in the endosome (72 ± 20.6 nM) than lysosome (9.4 ± 4.2 nM) after internalization. The demonstrated approach and findings not only lay the foundations for a quantitative understanding of the fate of nanoparticle-based targeting but also provide new insights into the rational design of nanoparticle delivery systems for effective treatment.

AB - Understanding the diffusion dynamics and receptor uptake mechanism of nanoparticles in cancer cells is crucial to the rational design of multifunctional nanoprobes for targeting and delivery. In this report, for the first time, we quantify the localization and evaluate the diffusion times of Herceptin-conjugated gold nanorods (H-GNRs) in different cell organelles by fluorescence correlation spectroscopy (FCS) and examine the endocytic diffusion of H-GNRs in live ErbB2 overexpressing SK-BR-3 cells. First, by colocalizing H-GNRs in different cellular organelles depicted by the respective markers, we demonstrate that H-GNRs colocalize with the endosome and lysosome but not with the Golgi apparatus. Our study shows that Herceptin-conjugated GNRs have similar intracellular localization characteristics as Herceptin - ErbB2 complex, with a higher concentration found in the endosome (72 ± 20.6 nM) than lysosome (9.4 ± 4.2 nM) after internalization. The demonstrated approach and findings not only lay the foundations for a quantitative understanding of the fate of nanoparticle-based targeting but also provide new insights into the rational design of nanoparticle delivery systems for effective treatment.

KW - Compartmentalization

KW - Endocytosis

KW - ErbB2

KW - Fluorescence correlation spectroscopy

KW - Herceptin - gold nanorods

KW - Quantification

UR - http://www.scopus.com/inward/record.url?scp=73849138217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73849138217&partnerID=8YFLogxK

U2 - 10.1021/nn900743v

DO - 10.1021/nn900743v

M3 - Article

C2 - 19891423

AN - SCOPUS:73849138217

VL - 3

SP - 4071

EP - 4079

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 12

ER -