Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

Jason Merritt; Seppe Kuehn

doi:10.1038/srep33173

Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

Jason Merritt, Seppe Kuehn

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

Original language	English (US)
Article number	33173
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep33173
State	Published - Sep 12 2016

ASJC Scopus subject areas

General

Online availability

10.1038/srep33173

Library availability

Discover UIUC Full Text

Cite this

@article{85e4a10a6878491f8bd933af53f2855d,

title = "Quantitative high-throughput population dynamics in continuous-culture by automated microscopy",

abstract = "We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.",

author = "Jason Merritt and Seppe Kuehn",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

month = sep,

day = "12",

doi = "10.1038/srep33173",

language = "English (US)",

volume = "6",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

AU - Merritt, Jason

AU - Kuehn, Seppe

N1 - Publisher Copyright: © The Author(s) 2016.

PY - 2016/9/12

Y1 - 2016/9/12

N2 - We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

AB - We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

UR - http://www.scopus.com/inward/record.url?scp=84987670204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987670204&partnerID=8YFLogxK

U2 - 10.1038/srep33173

DO - 10.1038/srep33173

M3 - Article

C2 - 27616752

AN - SCOPUS:84987670204

SN - 2045-2322

VL - 6

JO - Scientific reports

JF - Scientific reports

M1 - 33173

ER -

Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this