Quantitative confocal phase imaging by synthetic optical holography

M. Schnell, M. J. Perez-Roldan, P. S. Carney, R. Hillenbrand

Research output: Contribution to journalArticle

Abstract

We demonstrate quantitative phase mapping in confocal optical microscopy by applying synthetic optical holography (SOH), a recently introduced method for technically simple and fast phase imaging in scanning optical microscopy. SOH is implemented in a confocal microscope by simply adding a linearly moving reference mirror to the microscope setup, which generates a synthetic reference wave analogous to the plane reference wave of wide-field off-axis holography. We demonstrate that SOH confocal microscopy allows for non-contact surface profiling with sub-nanometer depth resolution. As an application for biological imaging, we apply SOH confocal microscopy to map the surface profile of an onion cell, revealing nanoscale-height features on the cell surface.

Original languageEnglish (US)
Pages (from-to)15267-15276
Number of pages10
JournalOptics Express
Volume22
Issue number12
DOIs
StatePublished - Jun 16 2014

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Schnell, M., Perez-Roldan, M. J., Carney, P. S., & Hillenbrand, R. (2014). Quantitative confocal phase imaging by synthetic optical holography. Optics Express, 22(12), 15267-15276. https://doi.org/10.1364/OE.22.015267