Quantitative analysis of modified proteins and their positional isomers by tandem mass spectrometry: Human histone H4

James J. Pesavento, Craig A. Mizzen, Neil L. Kelleher

Research output: Contribution to journalArticle

Abstract

Here we show that fragment ion abundances from dissociation of ions created from mixtures of multiply modified histone H4 (11 kDa) or of N-terminal synthetic peptides (2 kDa) correspond to their respective intact ion abundances measured by Fourier transform mass spectrometry. Isomeric mixtures of modified forms of the same protein are resolved and quantitated with a precision of ≤5% using the relative ratios of their fragment ions, with intact protein ions created by electrospray greatly easing many of the systematic biases that more strongly affect small peptides (e.g., differences in ionization efficiency and ion m/z values). The ion fragmentation methods validated here are directly extensible to intact human proteins to derive quantitative information on the highly related and often isomeric protein forms created by combinatorial arrays of posttranslational modifications.

Original languageEnglish (US)
Pages (from-to)4271-4280
Number of pages10
JournalAnalytical chemistry
Volume78
Issue number13
DOIs
StatePublished - Jul 1 2006

ASJC Scopus subject areas

  • Analytical Chemistry

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