TY - JOUR
T1 - Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)
AU - Zhuo, Yue
AU - Choi, Ji Sun
AU - Marin, Thibault
AU - Yu, Hojeong
AU - Harley, Brendan A.
AU - Cunningham, Brian T.
N1 - Funding Information:
This work is supported by the National Science Foundation (NSF) Grant CBET 11-32301 and National Institutes of Health (NIH) R01 DK099528 and NIH R21 EB018481. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NSF and NIH. The authors would like to thank the Nano Sensor Groups (NSG), the staff at the Beckman Institute for Advanced Science and Technology, the Micro and Nanotechnology Laboratory (MNTL), the Institute for Genomic Biology (IGB), and the Center for Innovative Instrumentation Technology (CiiT) at the University of Illinois at Urbana-Champaign for their support.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed. We utilized a label-free approach for dynamic, long-term, quantitative imaging of cell-surface interactions called photonic resonator outcoupler microscopy (PROM) in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor, resulting in a highly localized reduction of the reflected light intensity. By mapping the changes in the resonant reflected peak intensity from the biosensor surface, we demonstrate the ability of PROM to detect focal adhesion dimensions. Similar spatial distributions can be observed between PROM images and fluorescence-labeled images of focal adhesion areas in dental epithelial stem cells. In particular, we demonstrate that cell-surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously, providing a general-purpose tool for kinetic, high axial-resolution monitoring of cell interactions with basement membranes.
AB - Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed. We utilized a label-free approach for dynamic, long-term, quantitative imaging of cell-surface interactions called photonic resonator outcoupler microscopy (PROM) in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor, resulting in a highly localized reduction of the reflected light intensity. By mapping the changes in the resonant reflected peak intensity from the biosensor surface, we demonstrate the ability of PROM to detect focal adhesion dimensions. Similar spatial distributions can be observed between PROM images and fluorescence-labeled images of focal adhesion areas in dental epithelial stem cells. In particular, we demonstrate that cell-surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously, providing a general-purpose tool for kinetic, high axial-resolution monitoring of cell interactions with basement membranes.
UR - http://www.scopus.com/inward/record.url?scp=85047906291&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047906291&partnerID=8YFLogxK
U2 - 10.1038/s41377-018-0001-5
DO - 10.1038/s41377-018-0001-5
M3 - Article
C2 - 29963322
AN - SCOPUS:85047906291
VL - 7
JO - Light: Science and Applications
JF - Light: Science and Applications
SN - 2095-5545
IS - 1
M1 - 7538
ER -