Quantitative analysis of biological tissues using Fourier transform-second-harmonic generation imaging

Raghu Ambekar Ramachandra Rao, Monal R. Mehta, Kimani C. Toussaint

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the use of Fourier transform-second-harmonic generation (FT-SHG) imaging of collagen fibers as a means of performing quantitative analysis of obtained images of selected spatial regions in porcine trachea, ear, and cornea. Two quantitative markers, preferred orientation and maximum spatial frequency are proposed for differentiating structural information between various spatial regions of interest in the specimens. The ear shows consistent maximum spatial frequency and orientation as also observed in its real-space image. However, there are observable changes in the orientation and minimum feature size of fibers in the trachea indicating a more random organization. Finally, the analysis is applied to a 3D image stack of the cornea. It is shown that the standard deviation of the orientation is sensitive to the randomness in fiber orientation. Regions with variations in the maximum spatial frequency, but with relatively constant orientation, suggest that maximum spatial frequency is useful as an independent quantitative marker. We emphasize that FT-SHG is a simple, yet powerful, tool for extracting information from images that is not obvious in real space. This technique can be used as a quantitative biomarker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences X
DOIs
StatePublished - May 7 2010
EventMultiphoton Microscopy in the Biomedical Sciences X - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7569
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences X
CountryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

Keywords

  • Collagen
  • Image analysis
  • Second-harmonic generation microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ambekar Ramachandra Rao, R., Mehta, M. R., & Toussaint, K. C. (2010). Quantitative analysis of biological tissues using Fourier transform-second-harmonic generation imaging. In Multiphoton Microscopy in the Biomedical Sciences X [75692G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7569). https://doi.org/10.1117/12.841208