Quantitation of estrogen effects on Xenopus laevis albumin mRNA levels by hybridization to cloned albumin cDNA

B. W. Philipp, E. A. Morgan, D. J. Shapiro

Research output: Contribution to journalArticlepeer-review

Abstract

The isolation and characterization of a Xenopus laevis albumin cDNA clone, pUN18, is described. This clone was identified as a X. laevis albumin cDNA clone by hybridization-mRNA selection-translation and by other techniques. The clone was used in hybridizations to determine the effect of estrogen on albumin synthesis of livers of male X. laevis. We find that albumin mRNA levels remain relatively constant during the massive induction of vitellogenin mRNA synthesis and vitellogenin secretion which follow administration of estrogen to male X. laevis.

Original languageEnglish (US)
Pages (from-to)8496-8501
Number of pages6
JournalJournal of Biological Chemistry
Volume257
Issue number14
StatePublished - 1982

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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