Quantifying molecular- To cellular-level forces in living cells

Jason Pan, Tommy Kmieciak, Yen Ting Liu, Matthew Wildenradt, Yun Sheng Chen, Yang Zhao

Research output: Contribution to journalReview articlepeer-review


Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.

Original languageEnglish (US)
Article number483001
JournalJournal of Physics D: Applied Physics
Issue number48
StatePublished - Dec 2 2021


  • FRET
  • atomic force microscopy
  • conformational imaging
  • mechanobiology
  • molecular forces

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films


Dive into the research topics of 'Quantifying molecular- To cellular-level forces in living cells'. Together they form a unique fingerprint.

Cite this