Quantifying molecular- To cellular-level forces in living cells

Jason Pan; Tommy Kmieciak; Yen Ting Liu; Matthew Wildenradt; Yun Sheng Chen; Yang Zhao

doi:10.1088/1361-6463/ac2170

Quantifying molecular- To cellular-level forces in living cells

Jason Pan, Tommy Kmieciak, Yen Ting Liu, Matthew Wildenradt, Yun Sheng Chen, Yang Zhao

Research output: Contribution to journal › Review article › peer-review

Abstract

Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.

Original language	English (US)
Article number	483001
Journal	Journal of Physics D: Applied Physics
Volume	54
Issue number	48
DOIs	https://doi.org/10.1088/1361-6463/ac2170
State	Published - Dec 2 2021

Keywords

FRET
atomic force microscopy
conformational imaging
mechanobiology
molecular forces

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Acoustics and Ultrasonics
Surfaces, Coatings and Films

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10.1088/1361-6463/ac2170

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title = "Quantifying molecular- To cellular-level forces in living cells",

abstract = "Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.",

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author = "Jason Pan and Tommy Kmieciak and Liu, {Yen Ting} and Matthew Wildenradt and Chen, {Yun Sheng} and Yang Zhao",

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AU - Pan, Jason

AU - Kmieciak, Tommy

AU - Liu, Yen Ting

AU - Wildenradt, Matthew

AU - Chen, Yun Sheng

AU - Zhao, Yang

PY - 2021/12/2

Y1 - 2021/12/2

N2 - Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.

AB - Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.

KW - FRET

KW - atomic force microscopy

KW - conformational imaging

KW - mechanobiology

KW - molecular forces

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Quantifying molecular- To cellular-level forces in living cells

Abstract

Keywords

ASJC Scopus subject areas

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