Quantifying compressive forces between living cell layers and within tissues using elastic round microgels

Erfan Mohagheghian, Junyu Luo, Junjian Chen, Gaurav Chaudhary, Junwei Chen, Jian Sun, Randy H Ewoldt, Ning Wang

Research output: Contribution to journalArticle

Abstract

Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist that can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conjugated alginate hydrogels (elastic round microgels, ERMGs). We generate 3D displacements and calculate strains and tractions exerted on an ERMG. Average compressive tractions on an ERMG are 570 Pa within cell layers and 360 Pa in tumor-repopulating cell (TRC) colonies grown in 400-Pa matrices. 3D compressive tractions on a 1.4-kPa ERMG are applied by surrounding cells via endogenous actomyosin forces but not via mature focal adhesions. Compressive stresses are substantially heterogeneous on ERMGs within a uniform cell colony and do not increase with TRC colony sizes. Early-stage zebrafish embryos generate spatial and temporal differences in local normal and shear stresses. This ERMG method could be useful for quantifying stresses in vitro and in vivo.

Original languageEnglish (US)
Article number1878
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

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Elastic Tissue
Compressive stress
Tumors
Cells
Tissue
traction
Traction
Actomyosin
Traction (friction)
Hydrogels
cells
Microspheres
Shear stress
Adhesion
Nanoparticles
tumors
Mechanical Stress
Focal Adhesions
critical loading
embryos

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Quantifying compressive forces between living cell layers and within tissues using elastic round microgels. / Mohagheghian, Erfan; Luo, Junyu; Chen, Junjian; Chaudhary, Gaurav; Chen, Junwei; Sun, Jian; Ewoldt, Randy H; Wang, Ning.

In: Nature communications, Vol. 9, No. 1, 1878, 01.12.2018.

Research output: Contribution to journalArticle

Mohagheghian, Erfan ; Luo, Junyu ; Chen, Junjian ; Chaudhary, Gaurav ; Chen, Junwei ; Sun, Jian ; Ewoldt, Randy H ; Wang, Ning. / Quantifying compressive forces between living cell layers and within tissues using elastic round microgels. In: Nature communications. 2018 ; Vol. 9, No. 1.
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