Abstract
Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 14387-14394 |
| Number of pages | 8 |
| Journal | Analytical chemistry |
| Volume | 90 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 18 2018 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles. / Zhang, Xi; Pandiakumar, Arun Kumar; Hamers, Robert J.; Murphy, Catherine Jones.
In: Analytical chemistry, Vol. 90, No. 24, 18.12.2018, p. 14387-14394.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles
AU - Zhang, Xi
AU - Pandiakumar, Arun Kumar
AU - Hamers, Robert J.
AU - Murphy, Catherine Jones
PY - 2018/12/18
Y1 - 2018/12/18
N2 - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.
AB - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.
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UR - http://www.scopus.com/inward/citedby.url?scp=85057561803&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.8b03911
DO - 10.1021/acs.analchem.8b03911
M3 - Article
C2 - 30427176
AN - SCOPUS:85057561803
VL - 90
SP - 14387
EP - 14394
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 24
ER -