Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles

Xi Zhang, Arun Kumar Pandiakumar, Robert J. Hamers, Catherine Jones Murphy

Research output: Contribution to journalArticle

Abstract

Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.

Original languageEnglish (US)
Pages (from-to)14387-14394
Number of pages8
JournalAnalytical chemistry
Volume90
Issue number24
DOIs
StatePublished - Dec 18 2018

Fingerprint

Nanoparticles
Lipids
Surface chemistry
Liquid chromatography
Dynamic light scattering
Biological systems
Ammonium Compounds
Gold
Amines
Mass spectrometry
Electrostatics
Polymers
Fluids
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles. / Zhang, Xi; Pandiakumar, Arun Kumar; Hamers, Robert J.; Murphy, Catherine Jones.

In: Analytical chemistry, Vol. 90, No. 24, 18.12.2018, p. 14387-14394.

Research output: Contribution to journalArticle

Zhang, Xi ; Pandiakumar, Arun Kumar ; Hamers, Robert J. ; Murphy, Catherine Jones. / Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles. In: Analytical chemistry. 2018 ; Vol. 90, No. 24. pp. 14387-14394.
@article{384ae5181ec1427d8af16f8f59c4f0a6,
title = "Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles",
abstract = "Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a {"}pull-down{"} scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95{\%} of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.",
author = "Xi Zhang and Pandiakumar, {Arun Kumar} and Hamers, {Robert J.} and Murphy, {Catherine Jones}",
year = "2018",
month = "12",
day = "18",
doi = "10.1021/acs.analchem.8b03911",
language = "English (US)",
volume = "90",
pages = "14387--14394",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles

AU - Zhang, Xi

AU - Pandiakumar, Arun Kumar

AU - Hamers, Robert J.

AU - Murphy, Catherine Jones

PY - 2018/12/18

Y1 - 2018/12/18

N2 - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.

AB - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.

UR - http://www.scopus.com/inward/record.url?scp=85057561803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057561803&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.8b03911

DO - 10.1021/acs.analchem.8b03911

M3 - Article

C2 - 30427176

AN - SCOPUS:85057561803

VL - 90

SP - 14387

EP - 14394

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 24

ER -