TY - JOUR
T1 - Quantification of Lipid Corona Formation on Colloidal Nanoparticles from Lipid Vesicles
AU - Zhang, Xi
AU - Pandiakumar, Arun Kumar
AU - Hamers, Robert J.
AU - Murphy, Catherine J.
N1 - This work was supported by National Science Foundation under the Center for Sustainable Nanotechnology (CSN), CHE-1503408. The CSN is part of the Centers for Chemical Innovation Program. We thank CSN members for support, especially Professors Joel Pedersen and Christy Haynes. We thank Dr. Lucas Li, Metabolomics Laboratory of the Roy J. Carver Biotechnology Center at UIUC, for the raw data of lipid quantification.
PY - 2018/12/18
Y1 - 2018/12/18
N2 - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.
AB - Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions. Qualitative (plasmon band shifting, -potential analysis, dynamic light scattering on the part of the nanoparticles) and quantitative (lipid liquid chromatography/mass spectrometry) methods are developed with a "pull-down" scheme to assess the degree of lipid corona formation in these systems. In general, cationic nanoparticles extract 60-95% of the lipids available in vesicles under the described experimental conditions, while anionic nanoparticles extract almost none. While electrostatics apparently dominate the lipid-nanoparticle interactions, primary amine polymer surfaces extract more lipids than quaternary ammonium surfaces. Free cationic species can act as lipid-binding competitors in solution.
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U2 - 10.1021/acs.analchem.8b03911
DO - 10.1021/acs.analchem.8b03911
M3 - Article
C2 - 30427176
AN - SCOPUS:85057561803
SN - 0003-2700
VL - 90
SP - 14387
EP - 14394
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 24
ER -