Quantification of collagen fiber organization using three-dimensional Fourier transform-second-harmonic generation imaging

Tung Yuen Lau, Raghu Ambekar, Kimani C. Toussaint

Research output: Contribution to journalArticlepeer-review

Abstract

We present three-dimensional Fourier transform-second-harmonic generation (3D FT-SHG) imaging, a generalization of the previously reported two-dimensional FT-SHG, to quantify collagen fiber organization from 3D image stacks of biological tissues. The current implementation calculates 3D preferred orientation of a region of interest, and classifies regions of interest based on orientation anisotropy and average voxel intensity. Presented are some example applications of the technique which reveal the layered structure of collagen fibers in porcine sclera, and estimates the cut angle of porcine tendon tissues. This technique shows promising potential for studying biological tissues that contain fibrillar structures in 3D.

Original languageEnglish (US)
Pages (from-to)21821-21832
Number of pages12
JournalOptics Express
Volume20
Issue number19
DOIs
StatePublished - Sep 10 2012

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Quantification of collagen fiber organization using three-dimensional Fourier transform-second-harmonic generation imaging'. Together they form a unique fingerprint.

Cite this