Quantification and cell-to-cell variation of vascular endothelial growth factor receptors

P. I. Imoukhuede, Aleksander S. Popel

Research output: Contribution to journalArticle

Abstract

The vascular endothelial growth factor receptors (VEGFR) play a significant role in angiogenesis, the formation of new blood vessels from existing vasculature. Systems biology offers promising approaches to better understand angiogenesis by computational modeling the key molecular interactions in this process. Such modeling requires quantitative knowledge of cell surface density of pro-angiogenic receptors versus anti-angiogenic receptors, their regulation, and their cell-to-cell variability. Using quantitative fluorescence, we systematically characterized the endothelial surface density of VEGFRs and neuropilin-1 (NRP1). We also determined the role of VEGF in regulating the surface density of these receptors. Applying cell-by-cell analysis revealed heterogeneity in receptor surface density and VEGF tuning of this heterogeneity. Altogether, we determine inherent differences in the surface expression levels of these receptors and the role of VEGF in regulating the balance of anti-angiogenic or modulatory (VEGFR1) and pro-angiogenic (VEGFR2) receptors.

Original languageEnglish (US)
Pages (from-to)955-965
Number of pages11
JournalExperimental Cell Research
Volume317
Issue number7
DOIs
StatePublished - Apr 15 2011

Keywords

  • Angiogenesis
  • Flow cytometry
  • NRP1
  • Systems biology
  • VEGF

ASJC Scopus subject areas

  • Cell Biology

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