QTL mapping and epistatic interaction analysis of field resistance to sudden death syndrome (Fusarium virguliforme) in soybean

Ruijuan Tan, Bradley Serven, Paul J. Collins, Zhongnan Zhang, Zixiang Wen, John F. Boyse, Cuihua Gu, Martin I. Chilvers, Brian W Diers, Dechun Wang

Research output: Contribution to journalArticle

Abstract

Key message: Two interactive quantitative trait loci (QTLs) controlled the field resistance to sudden death syndrome (SDS) in soybean. The interaction between them was confirmed. Abstract: Sudden death syndrome (SDS), caused by Fusarium virguliforme, is a major disease of soybean [Glycine max (L.) Merr.] in the United States. Breeding for soybean resistance to SDS is the most cost-effective method to manage the disease. The objective of this study was to identify and characterize quantitative trait loci (QTLs) underlying field resistance to SDS in a recombinant inbred line population from the cross GD2422 × LD01-5907. This population was genotyped with 1786 polymorphic single nucleotide polymorphisms (SNPs) using SoySNP6 K iSelect BeadChip and evaluated for SDS resistance in a naturally infested field. Four SDS resistance QTLs were mapped on Chromosomes 4, 8, 12 and 18. The resistant parent, LD01-5907, contributed the resistance alleles for the QTLs on Chromosomes 8 and 18 (qSDS-8 and qSDS-18), while the other parent, GD2422, provided the resistance alleles for the QTLs on Chromosomes 4 and 12 (qSDS-4 and qSDS-12). The minor QTL on Chromosome 12 (qSDS-12) is novel. The QTL on Chromosomes 8 and 18 (qSDS-8 and qSDS-18) overlapped with two soybean cyst nematode resistance-related loci, Rhg4 and Rhg1, respectively. A significant interaction between qSDS-8 and qSDS-18 was detected by disease incidence. Individual effects together with the interaction effect explained around 70% of the phenotypic variance. The epistatic interaction of qSDS-8 and qSDS-18 was confirmed by the field performance across multiple years. Furthermore, the resistance alleles at qSDS-8 and qSDS-18 were demonstrated to be recessive. The SNP markers linked to these QTLs will be useful for marker-assisted breeding to enhance the SDS resistance.

Original languageEnglish (US)
Pages (from-to)1729-1740
Number of pages12
JournalTheoretical and Applied Genetics
Volume131
Issue number8
DOIs
StatePublished - Aug 1 2018

Fingerprint

Quantitative Trait Loci
Fusarium
Sudden Death
Soybeans
quantitative trait loci
soybeans
death
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 8
chromosomes
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 4
Alleles
alleles
single nucleotide polymorphism
Breeding
Single Nucleotide Polymorphism
Heterodera glycines
Fusarium virguliforme
breeding

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

QTL mapping and epistatic interaction analysis of field resistance to sudden death syndrome (Fusarium virguliforme) in soybean. / Tan, Ruijuan; Serven, Bradley; Collins, Paul J.; Zhang, Zhongnan; Wen, Zixiang; Boyse, John F.; Gu, Cuihua; Chilvers, Martin I.; Diers, Brian W; Wang, Dechun.

In: Theoretical and Applied Genetics, Vol. 131, No. 8, 01.08.2018, p. 1729-1740.

Research output: Contribution to journalArticle

Tan, Ruijuan ; Serven, Bradley ; Collins, Paul J. ; Zhang, Zhongnan ; Wen, Zixiang ; Boyse, John F. ; Gu, Cuihua ; Chilvers, Martin I. ; Diers, Brian W ; Wang, Dechun. / QTL mapping and epistatic interaction analysis of field resistance to sudden death syndrome (Fusarium virguliforme) in soybean. In: Theoretical and Applied Genetics. 2018 ; Vol. 131, No. 8. pp. 1729-1740.
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AU - Tan, Ruijuan

AU - Serven, Bradley

AU - Collins, Paul J.

AU - Zhang, Zhongnan

AU - Wen, Zixiang

AU - Boyse, John F.

AU - Gu, Cuihua

AU - Chilvers, Martin I.

AU - Diers, Brian W

AU - Wang, Dechun

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