QPI for prostate cancer diagnosis: Quantitative separation of Gleason grades 3 and 4

Shamira Sridharan, Virgilia Macias, Krishnarao Tangella, Andre Kajdacsy-Balla, Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


1 in 7 men receive a diagnosis of prostate cancer in their lifetime. The aggressiveness of the treatment plan adopted by the patient is strongly influenced by Gleason grade. Gleason grade is determined by the pathologist based on the level of glandular formation and complexity seen in the patient's biopsy. However, studies have shown that the disagreement rate between pathologists on Gleason grades 3 and 4 is high and this affects treatment options. We used quantitative phase imaging to develop an objective method for Gleason grading. Using the glandular solidity, which is the ratio of the area of the gland to a convex hull fit around it, and anisotropy of light scattered from the stroma immediately adjoining the gland, we were able to quantitatively separate Gleason grades 3 and 4 with 81% accuracy in 43 cases marked as difficult by pathologists.

Original languageEnglish (US)
Title of host publicationQuantitative Phase Imaging
EditorsYongKeun Park, Gabriel Popescu
ISBN (Electronic)9781628414264
StatePublished - 2015
Event1st Conference on Quantitative Phase Imaging, QPI 2015 - San Francisco, United States
Duration: Feb 7 2015Feb 10 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


Other1st Conference on Quantitative Phase Imaging, QPI 2015
Country/TerritoryUnited States
CitySan Francisco


  • Gleason grading
  • cancer diagnosis
  • interferometry
  • label-free imaging
  • microscopy
  • prostate cancer
  • quantitative phase imaging
  • spatial light interference microscopy
  • tissue microarray

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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