Purification of the creatine kinase isozymes of the green sunfish (Lepomis cyanellus) with Blue Sepharose CL-6B

Suzanne E. Fisher, Gregory S. Whitt

Research output: Contribution to journalArticlepeer-review

Abstract

Three homodimeric creatine kinase isozymes (A2, B2, and C2) of the green sunfish (Lepomis cyanellus) were purified by a combination of affinity chromatography, gel filtration, and preparative starch gel electrophoresis. The final preparations were isozymically pure and were used to elicit antibodies in rabbits. The use of the group-specific adsorbant Blue Sepharose CL-6B (Pharmacia) and specific elution conditions for creatine kinase facilitated purification. Fish creatine kinase isozymes are sensitive to denaturation and cannot be readily purified by procedures routinely used for mammalian creatine kinase isozymes.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalAnalytical Biochemistry
Volume94
Issue number1
DOIs
StatePublished - Apr 1 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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