Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2

Garry Taylor; Eric Vimr; Elspeth Garman; Graeme Laver

doi:10.1016/0022-2836(92)91069-2

Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2

Garry Taylor, Eric Vimr, Elspeth Garman, Graeme Laver

Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

The nanH genes of Vibrio cholerae and Salmonella typhimurium LT2 coding neuraminidase were cloned separately in Escherichia coli, and the expression products purified. Single crystals of the V. cholerae neuraminidase were obtained using the hanging drop vapour diffusion method with polyethylene glycol as precipitant at pH 7·2. The crystals belong to the orthorhombic space group P2₁2₁2₁, with unit cell dimensions a = 71·9 A ̊, b = 79·0 A ̊, c = 165·7 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 2·5 Å. Single crystals of the S. typhimurium neuraminidase were obtained by hanging drop with potassium phosphate as precipitant at pH 7·2. The crystals also belong to the orthorhombic space group P2₁2₁2₁, with unit cell dimensions a = 47·4 A ̊, b = 82.8 A ̊, c = 92·4 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 1·8 Å.

Original language	English (US)
Pages (from-to)	1287-1290
Number of pages	4
Journal	Journal of Molecular Biology
Volume	226
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(92)91069-2
State	Published - May 20 1992

Keywords

Salmonella
cholera
neuraminidase
sialidase

ASJC Scopus subject areas

Biophysics
Structural Biology
Molecular Biology

Online availability

10.1016/0022-2836(92)91069-2

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abstract = "The nanH genes of Vibrio cholerae and Salmonella typhimurium LT2 coding neuraminidase were cloned separately in Escherichia coli, and the expression products purified. Single crystals of the V. cholerae neuraminidase were obtained using the hanging drop vapour diffusion method with polyethylene glycol as precipitant at pH 7·2. The crystals belong to the orthorhombic space group P212121, with unit cell dimensions a = 71·9 A ̊, b = 79·0 A ̊, c = 165·7 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 2·5 {\AA}. Single crystals of the S. typhimurium neuraminidase were obtained by hanging drop with potassium phosphate as precipitant at pH 7·2. The crystals also belong to the orthorhombic space group P212121, with unit cell dimensions a = 47·4 A ̊, b = 82.8 A ̊, c = 92·4 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 1·8 {\AA}.",

keywords = "Salmonella, cholera, neuraminidase, sialidase",

author = "Garry Taylor and Eric Vimr and Elspeth Garman and Graeme Laver",

note = "Funding Information: suitable heavy-atom derivatives is in was supported by NIH grant AI23039 of Allergy and Infectious Diseases.",

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AU - Laver, Graeme

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PY - 1992/5/20

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N2 - The nanH genes of Vibrio cholerae and Salmonella typhimurium LT2 coding neuraminidase were cloned separately in Escherichia coli, and the expression products purified. Single crystals of the V. cholerae neuraminidase were obtained using the hanging drop vapour diffusion method with polyethylene glycol as precipitant at pH 7·2. The crystals belong to the orthorhombic space group P212121, with unit cell dimensions a = 71·9 A ̊, b = 79·0 A ̊, c = 165·7 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 2·5 Å. Single crystals of the S. typhimurium neuraminidase were obtained by hanging drop with potassium phosphate as precipitant at pH 7·2. The crystals also belong to the orthorhombic space group P212121, with unit cell dimensions a = 47·4 A ̊, b = 82.8 A ̊, c = 92·4 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 1·8 Å.

AB - The nanH genes of Vibrio cholerae and Salmonella typhimurium LT2 coding neuraminidase were cloned separately in Escherichia coli, and the expression products purified. Single crystals of the V. cholerae neuraminidase were obtained using the hanging drop vapour diffusion method with polyethylene glycol as precipitant at pH 7·2. The crystals belong to the orthorhombic space group P212121, with unit cell dimensions a = 71·9 A ̊, b = 79·0 A ̊, c = 165·7 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 2·5 Å. Single crystals of the S. typhimurium neuraminidase were obtained by hanging drop with potassium phosphate as precipitant at pH 7·2. The crystals also belong to the orthorhombic space group P212121, with unit cell dimensions a = 47·4 A ̊, b = 82.8 A ̊, c = 92·4 A ̊, and with one molecule in the asymmetric unit. Diffraction extends to at least 1·8 Å.

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KW - sialidase

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Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2

Abstract

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