TY - JOUR
T1 - Purification and partial characterization of canine pepsinogen A and B
AU - Suchodolski, Jan S.
AU - Steiner, Jörg M.
AU - Ruaux, Craig G.
AU - Boari, Andrea
AU - Williams, David A.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Objective - To purify and partially characterize various isoforms of canine pepsinogen (PG) from gastric mucosa. Sample Population - Stomachs obtained from 6 euthanatized dogs. Procedure - Mucosa was scraped from canine stomachs, and a crude mucosal extract was prepared and further purified by use of weak anion-exchange chromatography, hydroxyapatite chromatography, size-exclusion chromatography, and strong anion-exchange chromatography. Pepsinogens were characterized by estimation of molecular weights, estimation of their isoelectric points (IEPs), and N-terminal amino acid sequencing. Results - Two different groups of canine PG were identified after the final strong anion-exchange chromatography: PG A and PG B. Pepsinogens differed in their molecular weights and IEP Pepsinogen B appeared to be a dinner with a molecular weight of approximately 34,100 and an IEP of 4.9. Pepsinogen A separated into several isoforms. Molecular weights for the various isoforms of PG A ranged from 34,200 to 42,100, and their IEPs ranged from 4.0 to < 3.0.The N-terminal amino acid sequence for the first 25 amino acid residues for PG A and B had good homology with the amino acid sequences for these proteins in other species. Conclusions and Clinical Relevance - Canine PG B and several isoforms of canine PG A have been purified. Availability of these PGs will facilitate development of immunoassays to measure PG in canine serum as a potential diagnostic marker for gastric disorders in dogs.
AB - Objective - To purify and partially characterize various isoforms of canine pepsinogen (PG) from gastric mucosa. Sample Population - Stomachs obtained from 6 euthanatized dogs. Procedure - Mucosa was scraped from canine stomachs, and a crude mucosal extract was prepared and further purified by use of weak anion-exchange chromatography, hydroxyapatite chromatography, size-exclusion chromatography, and strong anion-exchange chromatography. Pepsinogens were characterized by estimation of molecular weights, estimation of their isoelectric points (IEPs), and N-terminal amino acid sequencing. Results - Two different groups of canine PG were identified after the final strong anion-exchange chromatography: PG A and PG B. Pepsinogens differed in their molecular weights and IEP Pepsinogen B appeared to be a dinner with a molecular weight of approximately 34,100 and an IEP of 4.9. Pepsinogen A separated into several isoforms. Molecular weights for the various isoforms of PG A ranged from 34,200 to 42,100, and their IEPs ranged from 4.0 to < 3.0.The N-terminal amino acid sequence for the first 25 amino acid residues for PG A and B had good homology with the amino acid sequences for these proteins in other species. Conclusions and Clinical Relevance - Canine PG B and several isoforms of canine PG A have been purified. Availability of these PGs will facilitate development of immunoassays to measure PG in canine serum as a potential diagnostic marker for gastric disorders in dogs.
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U2 - 10.2460/ajvr.2002.63.1585
DO - 10.2460/ajvr.2002.63.1585
M3 - Article
C2 - 12428671
AN - SCOPUS:0036847482
SN - 0002-9645
VL - 63
SP - 1585
EP - 1590
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 11
ER -