Purification and comparison of corticosteroid-induced and intestinal isoenzymes of alkaline phosphatase in dogs.

R. K. Sanecki, W. E. Hoffmann, J. L. Dorner, M. S. Kuhlenschmidt

Research output: Contribution to journalArticlepeer-review

Abstract

Corticosteroid-induced alkaline phosphatase (CALP) and intestinal alkaline phosphatase (IALP) from dogs were purified to homogeneity, as determined by polyacrylamide gel electrophoresis. Purification involved an un-interrupted system using DEAE-cellulose, concanavalin A-agarose, and monoclonal antibody affinity columns. The monoclonal antibody was prepared by use of IALP as the antigen. The 2 isoenzymes were compared, using molecular weight determinations, amino acid analyses, peptide mapping, N-terminal sequencing of the first 10 amino acids, carbohydrate analyses, and recognition by anti-IALP monoclonal antibody. The data indicated that canine IALP and CALP are identical with regard to recognition by monoclonal antibody and N-terminal amino acid sequence, nearly identical in amino acid content and peptide maps, but different in carbohydrate content. It was concluded that CALP is a product of the same gene as IALP and that differences in glycosyl transferase activities between liver and intestines or the presence of glycosidase activities in or around the intestinal mucosae result in the marked difference in carbohydrate content.

Original languageEnglish (US)
Pages (from-to)1964-1968
Number of pages5
JournalAmerican journal of veterinary research
Volume51
Issue number12
StatePublished - Dec 1990

ASJC Scopus subject areas

  • veterinary(all)

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