Pumpless microfluidic devices for generating healthy and diseased endothelia

Yang Yang, Parinaz Fathi, Glenn Holland, Dipanjan Pan, Nam Sun Wang, Mandy B. Esch

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a pumpless cell culture chip that can recirculate small amounts of cell culture medium (400 μL) in a unidirectional flow pattern. When operated with the accompanying custom rotating platform, the device produces an average wall shear stress of up to 0.588 Pa ± 0.006 Pa without the use of a pump. It can be used to culture cells that are sensitive to the direction of flow-induced mechanical shear such as human umbilical vein endothelial cells (HUVECs) in a format that allows for large-scale parallel screening of drugs. Using the device we demonstrate that HUVECs produce pro-inflammatory indicators (interleukin 6, interleukin 8) under both unidirectional and bidirectional flow conditions, but that the secretion was significantly lower under unidirectional flow. Our results show that pumpless devices can simulate the endothelium under healthy and activated conditions. The developed devices can be integrated with pumpless tissues-on-chips, allowing for the addition of barrier tissues such as endothelial linings.

Original languageEnglish (US)
Pages (from-to)3212-3219
Number of pages8
JournalLab on a chip
Volume19
Issue number19
DOIs
StatePublished - Oct 7 2019

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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