Pulsed-field gel electrophoresis does not break E. coli chromosome undergoing excision repair after UV irradiation

Research output: Contribution to journalArticlepeer-review

Abstract

We showed before that long linear DNA molecules containing single-strand interruptions and undergoing pulsed-field gel electrophoresis (PFGE) tend to break into subfragments (electrophoretic nick instability). Here we show that circular chromosomal DNA with single-strand interruptions remains in the wells during PFGE. This means that the presence of nicks in immobile circular DNA is not enough to break this DNA during PFGE. In other words, under the conditions of our study, the artifactual conversion of nicks into double-strand breaks that we detect in linear DNA does not contribute to the overall level of chromosomal fragmentation, as measured by PFGE.

Original languageEnglish (US)
Pages (from-to)66-68
Number of pages3
JournalAnalytical Biochemistry
Volume526
DOIs
StatePublished - Jun 1 2017

Keywords

  • Double-strand breaks
  • Nucleotide excision repair
  • Single-strand breaks
  • Ultraviolet light

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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