Pulse shaping strategies for nonlinear interferometric vibrational imaging optimized for biomolecular imaging

Daniel L. Marks, Claudio Vinegoni, Jeremy S. Bredfeldt, Stephen A. Boppart

Research output: Contribution to journalConference article

Abstract

Nonlinear Interferometric Vibrational Imaging (NIVI) measures the temporal cross-correlation of anti-Stokes radiation from Coherent anti-Stokes Raman Scattering (CARS) processes to achieve increased sensitivity, stray light rejection, and nonresonant background rejection. Because the intensity of CARS radiation is proportional to the square of the molecular density of a target resonance, it is critical to maximize the recoverable signal for a given illumination level. Especially if one desires to measure several resonances, there can be a sensitivity as well as a speed advantage to measuring them simultaneously rather than serially. We discuss the methods of sample excitation that NIVI allows and their potential sensitivity advantages, as well as present experimental results demonstrating Raman signal recovery using these pulse sequences.

Original languageEnglish (US)
Pages (from-to)5300-5303
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 VII
StatePublished - Dec 1 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

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ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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