Pseudomonas aeruginosa pyocyanin modulates mucin glycosylation with sialyl-Lewis x to increase binding to airway epithelial cells

J. L. Jeffries, J. Jia, W. Choi, S. Choe, J. Miao, Y. Xu, R. Powell, J. Lin, Z. Kuang, H Rex Gaskins, Gee Lau

Research output: Contribution to journalArticle

Abstract

Cystic fibrosis (CF) patients battle life-long pulmonary infections with the respiratory pathogen Pseudomonas aeruginosa (PA). An overabundance of mucus in CF airways provides a favorable niche for PA growth. When compared with that of non-CF individuals, mucus of CF airways is enriched in sialyl-Lewis x, a preferred binding receptor for PA. Notably, the levels of sialyl-Lewis x directly correlate with infection severity in CF patients. However, the mechanism by which PA causes increased sialylation remains uncharacterized. In this study, we examined the ability of PA virulence factors to modulate sialyl-Lewis x modification in airway mucins. We found pyocyanin (PCN) to be a potent inducer of sialyl-Lewis x in both mouse airways and in primary and immortalized CF and non-CF human airway epithelial cells. PCN increased the expression of C2/4GnT and ST3Gal-IV, two of the glycosyltransferases responsible for the stepwise biosynthesis of sialyl-Lewis x, through a tumor necrosis factor (TNF)-α-mediated phosphoinositol-specific phospholipase C (PI-PLC)-dependent pathway. Furthermore, PA bound more efficiently to airway epithelial cells pre-exposed to PCN in a flagellar cap-dependent manner. Importantly, antibodies against sialyl-Lewis x and anti-TNF-α attenuated PA binding. These results indicate that PA secretes PCN to induce a favorable environment for chronic colonization of CF lungs by increasing the glycosylation of airway mucins with sialyl-Lewis x.

Original languageEnglish (US)
Pages (from-to)1039-1050
Number of pages12
JournalMucosal Immunology
Volume9
Issue number4
DOIs
StatePublished - Jul 1 2016

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Pyocyanine
Mucins
Glycosylation
Pseudomonas aeruginosa
Epithelial Cells
Cystic Fibrosis
beta-galactoside alpha-2,3-sialyltransferase
Mucus
Fibrosis
Tumor Necrosis Factor-alpha
Phosphoinositide Phospholipase C
Glycosyltransferases
Lung
5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine
Virulence Factors
Respiratory Tract Infections

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Pseudomonas aeruginosa pyocyanin modulates mucin glycosylation with sialyl-Lewis x to increase binding to airway epithelial cells. / Jeffries, J. L.; Jia, J.; Choi, W.; Choe, S.; Miao, J.; Xu, Y.; Powell, R.; Lin, J.; Kuang, Z.; Gaskins, H Rex; Lau, Gee.

In: Mucosal Immunology, Vol. 9, No. 4, 01.07.2016, p. 1039-1050.

Research output: Contribution to journalArticle

Jeffries, J. L. ; Jia, J. ; Choi, W. ; Choe, S. ; Miao, J. ; Xu, Y. ; Powell, R. ; Lin, J. ; Kuang, Z. ; Gaskins, H Rex ; Lau, Gee. / Pseudomonas aeruginosa pyocyanin modulates mucin glycosylation with sialyl-Lewis x to increase binding to airway epithelial cells. In: Mucosal Immunology. 2016 ; Vol. 9, No. 4. pp. 1039-1050.
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