PrPd accumulation in organs of ARQ/ARQ sheep experimentally infected with BSE by peripheral routes

Stéphane Lezmi, Frédéric Ronzon, Anna Bencsik, Alexandre Bedin, Didier Calavas, Yves Richard, Stéphanie Simon, Jacques Grassi, Thierry Baron

Research output: Contribution to journalArticlepeer-review

Abstract

To study the pathogenesis of bovine spongiform encephalopathy infection in small ruminants, two Lacaune sheep with the AA136RR154QQ171 and one with the AA136RR154RR171 genotype for the prion protein, were inoculated with a brain homogenate from a French cattle BSE case by peripheral routes. Sheep with the ARQ/ARQ genotype are considered as susceptible to prion diseases contrary to those with the ARR/ARR genotype. The accumulation of disease-associated prion protein (PrPd) was analysed by biochemical and immunohistochemical methods. No PrPd accumulation was detected in samples from the ARR/ARR sheep 2 years post inoculation. In the two ARQ/ARQ sheep that had scrapie-like clinical symptoms, PrPd was found in the central, sympathetic and enteric nervous systems and in lymphoid organs. Remarkably, PrPd was also detected in some muscle types as well as in all peripheral nerves that had not been reported previously thus revealing a widespread distribution of BSE-associated PrPd in sheep tissues.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalActa Biochimica Polonica
Volume53
Issue number2
DOIs
StatePublished - 2006
Externally publishedYes

Keywords

  • Ovine BSE
  • Prion
  • Scrapie
  • Sheep

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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