Protozoal-related mortalities in endangered Hawaiian monk seals Neomonachus schauinslandi

Michelle M. Barbieri, Lizabeth Kashinsky, David S. Rotstein, Kathleen M. Colegrove, Katherine H. Haman, Spencer L. Magargal, Amy R. Sweeny, Angela C. Kaufman, Michael E. Grigg, Charles L. Littnan

Research output: Contribution to journalArticle

Abstract

Protozoal infections have been widely documented in marine mammals and may cause morbidity and mortality at levels that result in population level effects. The presence and potential impact on the recovery of endangered Hawaiian monk seals Neomonachus schauinslandi by protozoal pathogens was first identified in the carcass of a stranded adult male with disseminated toxoplasmosis and a captive monk seal with hepatitis. We report 7 additional cases and 2 suspect cases of protozoal-related mortality in Hawaiian monk seals between 2001 and 2015, including the first record of vertical transmission in this species. This study establishes case definitions for classification of protozoal infections in Hawaiian monk seals. Histopathology and immunohistochemistry were the primary diagnostic modalities used to define cases, given that these analyses establish a direct link between disease and pathogen presence. Findings were supported by serology and molecular data when available. Toxoplasma gondii was the predominant apicomplexan parasite identified and was associated with 100% of mortalities (n = 8) and 50% of suspect cases (n = 2). Incidental identification of sarcocysts in the skeletal muscle without tissue inflammation occurred in 4 seals, including one co-infected with T. gondii. In 2015, 2 cases of toxo - plasmosis were identified ante-mortem and shared similar clinical findings, including hematological abnormalities and histopathology. Protozoal-related mortalities, specifically due to toxoplasmosis, are emerging as a threat to the recovery of this endangered pinniped and other native Hawaiian taxa. By establishing case definitions, this study provides a foundation for measuring the impact of these diseases on Hawaiian monk seals.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalDiseases of Aquatic Organisms
Volume121
Issue number2
DOIs
StatePublished - Sep 26 2016

Keywords

  • Immunohistochemistry
  • Mortality
  • Pathology
  • Pinniped
  • Protozoa
  • Sarco cystis
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Fingerprint Dive into the research topics of 'Protozoal-related mortalities in endangered Hawaiian monk seals Neomonachus schauinslandi'. Together they form a unique fingerprint.

  • Cite this

    Barbieri, M. M., Kashinsky, L., Rotstein, D. S., Colegrove, K. M., Haman, K. H., Magargal, S. L., Sweeny, A. R., Kaufman, A. C., Grigg, M. E., & Littnan, C. L. (2016). Protozoal-related mortalities in endangered Hawaiian monk seals Neomonachus schauinslandi. Diseases of Aquatic Organisms, 121(2), 85-95. https://doi.org/10.3354/dao03047