Proton-sponge coated quantum dots for siRNA delivery and intracellular imaging

Maksym V. Yezhelyev, Lifeng Qi, Ruth M. O'Regan, Shuming Nie, Xiaohu Gao

Research output: Contribution to journalArticlepeer-review

Abstract

We report the rational design of multifunctional nanoparticles for short-interfering RNA (siRNA) delivery and imaging based on the use of semiconductor quantum dots (QDs) and proton-absorbing polymeric coatings (proton sponges). With a balanced composition of tertiary amine and carboxylic acid groups, these nanoparticles are specifically designed to address longstanding barriers in siRNA delivery such as cellular penetration, endosomal release, carrier unpacking, and intracellular transport. The results demonstrate dramatic improvement in gene silencing efficiency by 10-20-fold and simultaneous reduction in cellular toxicity by 5-6-fold, when compared directly with existing transfection agents for MDA-MB-231 cells. The QD-siRNA nanoparticles are also dual-modality optical and electron-microscopy probes, allowing real-time tracking and ultrastructural localization of QDs during delivery and transfection. These new insights and capabilities represent a major step toward nanoparticle engineering for imaging and therapeutic applications.

Original languageEnglish (US)
Pages (from-to)9006-9012
Number of pages7
JournalJournal of the American Chemical Society
Volume130
Issue number28
DOIs
StatePublished - Jul 16 2008
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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