Proton-detected solid-state NMR spectroscopy of natural-abundance peptide and protein pharmaceuticals

Donghua H. Zhou, Gautam Shah, Charles Mullen, Dennis Sandoz, Chad M. Rienstra

Research output: Contribution to journalArticlepeer-review

Abstract

The natural way: A sensitive NMR spectroscopic method is developed to obtain well-resolved two-dimensional spectra (15N-1H and 13C-1H) for natural-abundance (that is, without the need for isotopic enrichment) large-molecule samples, such as biopharmaceuticals. This method gives structural insights on two lyophilized aprotinin samples and three insulin samples in lyophilized, microcrystalline suspension formulation (red; see picture) and fibril (green) forms.

Original languageEnglish (US)
Pages (from-to)1253-1256
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number7
DOIs
StatePublished - Feb 2 2009

Keywords

  • Insulin
  • Magic-angle spinning
  • NMR spectroscopy
  • Proteins
  • Structure elucidation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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