Abstract
NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).
| Original language | English (US) |
|---|---|
| Article number | 100894 |
| Journal | STAR Protocols |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 17 2021 |
Keywords
- Cell Biology
- Immunology
- Microbiology
- Microscopy
- Molecular Biology
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine