Abstract
Patients suffering from cystic fibrosis (CF) commonly harbor the important pathogen Pseudomonas aeruginosa in their airways. During chronic late-stage CF, P. aeruginosa is known to grow under reduced oxygen tension and is even capable of respiring anaerobically within the thickened airway mucus, at a pH of ∼6.5. Therefore, proteins involved in anaerobic metabolism represent potentially important targets for therapeutic intervention. In this study, the clinically relevant "anaerobiome" or "proteogenome" of P. aeruginosa was assessed. First, two different proteomic approaches were used to identify proteins differentially expressed under anaerobic versus aerobic conditions. Microarray studies were also performed, and in general, the anaerobic transcriptome was in agreement with the proteomic results. However, we found that a major portion of the most upregulated genes in the presence of NO3- and NO2- are those encoding Pf1 bacteriophage. With anaerobic NO2-, the most downregulated genes are those involved postglycolytically and include many tricarboxylic acid cycle genes and those involved in the electron transport chain, especially those encoding the NADH dehydrogenase I complex. Finally, a signature-tagged mutagenesis library of P. aeruginosa was constructed to further screen genes required for both NO3- and NO 2- respiration. In addition to genes anticipated to play important roles in the anaerobiome (anr, dnr, nar, nir, and nuo), the cysG and dksA genes were found to be required for both anaerobic NO3 - and NO2- respiration. This study represents a major step in unraveling the molecular machinery involved in anaerobic NO 3- and NO2- respiration and offers clues as to how we might disrupt such pathways in P. aeruginosa to limit the growth of this important CF pathogen when it is either limited or completely restricted in its oxygen supply.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2739-2758 |
| Number of pages | 20 |
| Journal | Journal of bacteriology |
| Volume | 190 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 1 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
Cite this
Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. / Platt, Mark D.; Schurr, Michael J.; Sauer, Karin; Vazquez, Gustavo; Kukavica-Ibrulj, Irena; Potvin, Eric; Levesque, Roger C.; Fedynak, Amber; Brinkman, Fiona S.L.; Schurr, Jill; Hwang, Sung Hei; Lau, Gee W.; Limbach, Patrick A.; Rowe, John J.; Lieberman, Michael A.; Barraud, Nicolas; Webb, Jeremy; Kjelleberg, Staffan; Hunt, Donald F.; Hassett, Daniel J.
In: Journal of bacteriology, Vol. 190, No. 8, 01.04.2008, p. 2739-2758.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions
AU - Platt, Mark D.
AU - Schurr, Michael J.
AU - Sauer, Karin
AU - Vazquez, Gustavo
AU - Kukavica-Ibrulj, Irena
AU - Potvin, Eric
AU - Levesque, Roger C.
AU - Fedynak, Amber
AU - Brinkman, Fiona S.L.
AU - Schurr, Jill
AU - Hwang, Sung Hei
AU - Lau, Gee W.
AU - Limbach, Patrick A.
AU - Rowe, John J.
AU - Lieberman, Michael A.
AU - Barraud, Nicolas
AU - Webb, Jeremy
AU - Kjelleberg, Staffan
AU - Hunt, Donald F.
AU - Hassett, Daniel J.
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Patients suffering from cystic fibrosis (CF) commonly harbor the important pathogen Pseudomonas aeruginosa in their airways. During chronic late-stage CF, P. aeruginosa is known to grow under reduced oxygen tension and is even capable of respiring anaerobically within the thickened airway mucus, at a pH of ∼6.5. Therefore, proteins involved in anaerobic metabolism represent potentially important targets for therapeutic intervention. In this study, the clinically relevant "anaerobiome" or "proteogenome" of P. aeruginosa was assessed. First, two different proteomic approaches were used to identify proteins differentially expressed under anaerobic versus aerobic conditions. Microarray studies were also performed, and in general, the anaerobic transcriptome was in agreement with the proteomic results. However, we found that a major portion of the most upregulated genes in the presence of NO3- and NO2- are those encoding Pf1 bacteriophage. With anaerobic NO2-, the most downregulated genes are those involved postglycolytically and include many tricarboxylic acid cycle genes and those involved in the electron transport chain, especially those encoding the NADH dehydrogenase I complex. Finally, a signature-tagged mutagenesis library of P. aeruginosa was constructed to further screen genes required for both NO3- and NO 2- respiration. In addition to genes anticipated to play important roles in the anaerobiome (anr, dnr, nar, nir, and nuo), the cysG and dksA genes were found to be required for both anaerobic NO3 - and NO2- respiration. This study represents a major step in unraveling the molecular machinery involved in anaerobic NO 3- and NO2- respiration and offers clues as to how we might disrupt such pathways in P. aeruginosa to limit the growth of this important CF pathogen when it is either limited or completely restricted in its oxygen supply.
AB - Patients suffering from cystic fibrosis (CF) commonly harbor the important pathogen Pseudomonas aeruginosa in their airways. During chronic late-stage CF, P. aeruginosa is known to grow under reduced oxygen tension and is even capable of respiring anaerobically within the thickened airway mucus, at a pH of ∼6.5. Therefore, proteins involved in anaerobic metabolism represent potentially important targets for therapeutic intervention. In this study, the clinically relevant "anaerobiome" or "proteogenome" of P. aeruginosa was assessed. First, two different proteomic approaches were used to identify proteins differentially expressed under anaerobic versus aerobic conditions. Microarray studies were also performed, and in general, the anaerobic transcriptome was in agreement with the proteomic results. However, we found that a major portion of the most upregulated genes in the presence of NO3- and NO2- are those encoding Pf1 bacteriophage. With anaerobic NO2-, the most downregulated genes are those involved postglycolytically and include many tricarboxylic acid cycle genes and those involved in the electron transport chain, especially those encoding the NADH dehydrogenase I complex. Finally, a signature-tagged mutagenesis library of P. aeruginosa was constructed to further screen genes required for both NO3- and NO 2- respiration. In addition to genes anticipated to play important roles in the anaerobiome (anr, dnr, nar, nir, and nuo), the cysG and dksA genes were found to be required for both anaerobic NO3 - and NO2- respiration. This study represents a major step in unraveling the molecular machinery involved in anaerobic NO 3- and NO2- respiration and offers clues as to how we might disrupt such pathways in P. aeruginosa to limit the growth of this important CF pathogen when it is either limited or completely restricted in its oxygen supply.
UR - http://www.scopus.com/inward/record.url?scp=41949140920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41949140920&partnerID=8YFLogxK
U2 - 10.1128/JB.01683-07
DO - 10.1128/JB.01683-07
M3 - Article
C2 - 18203836
AN - SCOPUS:41949140920
VL - 190
SP - 2739
EP - 2758
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 8
ER -