Proteome analysis of Methanococcus jannaschii by μLC/MS/MS

Wenhong Zhu; John R. Yates; Carol S. Giometti; Claudia I. Reich; Gary J Olsen

Proteome analysis of Methanococcus jannaschii by μLC/MS/MS

Wenhong Zhu, John R. Yates, Carol S. Giometti, Claudia I. Reich, Gary J Olsen

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Abstract

The application of μLC/MS/MS methods for the analysis of Methanococcus jannaschii proteome, was discussed. A 2D gel-based and a non-gel based method were used for the analysis. In the gel-based approach, two different protein specific strains and two different first dimension separation methods were used. In non-gel based approach, protein mixtures were first digested into peptides and loaded into a multidimensional chromatographic capillary column, and then separated and resolved by LC/LC/tandem mass spectrometry. The combination of multiple experimental results of MuPIT from both soluble and insoluble fractions of M. jannaschii resulted in more than 1100 unique protein identified, which represent about 65% of the Mj proteome.

Original language	English (US)
Pages	233-234
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

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Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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Spectroscopy

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title = "Proteome analysis of Methanococcus jannaschii by μLC/MS/MS",

abstract = "The application of μLC/MS/MS methods for the analysis of Methanococcus jannaschii proteome, was discussed. A 2D gel-based and a non-gel based method were used for the analysis. In the gel-based approach, two different protein specific strains and two different first dimension separation methods were used. In non-gel based approach, protein mixtures were first digested into peptides and loaded into a multidimensional chromatographic capillary column, and then separated and resolved by LC/LC/tandem mass spectrometry. The combination of multiple experimental results of MuPIT from both soluble and insoluble fractions of M. jannaschii resulted in more than 1100 unique protein identified, which represent about 65% of the Mj proteome.",

author = "Wenhong Zhu and Yates, {John R.} and Giometti, {Carol S.} and Reich, {Claudia I.} and Olsen, {Gary J}",

year = "2002",

language = "English (US)",

pages = "233--234",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Proteome analysis of Methanococcus jannaschii by μLC/MS/MS

AU - Zhu, Wenhong

AU - Yates, John R.

AU - Giometti, Carol S.

AU - Reich, Claudia I.

AU - Olsen, Gary J

PY - 2002

Y1 - 2002

N2 - The application of μLC/MS/MS methods for the analysis of Methanococcus jannaschii proteome, was discussed. A 2D gel-based and a non-gel based method were used for the analysis. In the gel-based approach, two different protein specific strains and two different first dimension separation methods were used. In non-gel based approach, protein mixtures were first digested into peptides and loaded into a multidimensional chromatographic capillary column, and then separated and resolved by LC/LC/tandem mass spectrometry. The combination of multiple experimental results of MuPIT from both soluble and insoluble fractions of M. jannaschii resulted in more than 1100 unique protein identified, which represent about 65% of the Mj proteome.

AB - The application of μLC/MS/MS methods for the analysis of Methanococcus jannaschii proteome, was discussed. A 2D gel-based and a non-gel based method were used for the analysis. In the gel-based approach, two different protein specific strains and two different first dimension separation methods were used. In non-gel based approach, protein mixtures were first digested into peptides and loaded into a multidimensional chromatographic capillary column, and then separated and resolved by LC/LC/tandem mass spectrometry. The combination of multiple experimental results of MuPIT from both soluble and insoluble fractions of M. jannaschii resulted in more than 1100 unique protein identified, which represent about 65% of the Mj proteome.

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M3 - Paper

AN - SCOPUS:2442673040

SP - 233

EP - 234

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteome analysis of Methanococcus jannaschii by μLC/MS/MS

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