Proteome analysis of Methanococcus jannaschii by μLC/MS/MS

Wenhong Zhu, John R. Yates, Carol S. Giometti, Claudia I. Reich, Gary J Olsen

Research output: Contribution to conferencePaper

Abstract

The application of μLC/MS/MS methods for the analysis of Methanococcus jannaschii proteome, was discussed. A 2D gel-based and a non-gel based method were used for the analysis. In the gel-based approach, two different protein specific strains and two different first dimension separation methods were used. In non-gel based approach, protein mixtures were first digested into peptides and loaded into a multidimensional chromatographic capillary column, and then separated and resolved by LC/LC/tandem mass spectrometry. The combination of multiple experimental results of MuPIT from both soluble and insoluble fractions of M. jannaschii resulted in more than 1100 unique protein identified, which represent about 65% of the Mj proteome.

Original languageEnglish (US)
Pages233-234
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Zhu, W., Yates, J. R., Giometti, C. S., Reich, C. I., & Olsen, G. J. (2002). Proteome analysis of Methanococcus jannaschii by μLC/MS/MS. 233-234. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.