Protein synthesis with isolated mitochondrial polysomes

N. G. Avadhani; D. E. Buetow

doi:10.1016/S0006-291X(72)80207-9

Protein synthesis with isolated mitochondrial polysomes

N. G. Avadhani, D. E. Buetow

Molecular and Integrative Physiology

Research output: Contribution to journal › Article › peer-review

Abstract

A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template. It is, thus, evident that mitochondria contain active messengers in the process of translation. The translational machinery in this organelle is prokaryotic in nature.

Original language	English (US)
Pages (from-to)	773-778
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(72)80207-9
State	Published - Jan 31 1972

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(72)80207-9

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title = "Protein synthesis with isolated mitochondrial polysomes",

abstract = "A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template. It is, thus, evident that mitochondria contain active messengers in the process of translation. The translational machinery in this organelle is prokaryotic in nature.",

author = "Avadhani, {N. G.} and Buetow, {D. E.}",

note = "Funding Information: This investigation was supported by a grant from the National Institute of Child Health and Human Development, HD-03163. We are grateful to Dr. J. M. Clark, Jr. for his useful advice and suggestions.",

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PY - 1972/1/31

Y1 - 1972/1/31

N2 - A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template. It is, thus, evident that mitochondria contain active messengers in the process of translation. The translational machinery in this organelle is prokaryotic in nature.

AB - A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template. It is, thus, evident that mitochondria contain active messengers in the process of translation. The translational machinery in this organelle is prokaryotic in nature.

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Protein synthesis with isolated mitochondrial polysomes

Abstract

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