Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM)

Yue Zhuo, Limei Tian, Weili Chen, Hojeong Yu, Srikanth Singamaneni, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.

Original languageEnglish (US)
Title of host publication2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2069-2072
Number of pages4
ISBN (Electronic)9781424479290
DOIs
StatePublished - Nov 2 2014
Event2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 - Chicago, United States
Duration: Aug 26 2014Aug 30 2014

Publication series

Name2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014

Other

Other2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
CountryUnited States
CityChicago
Period8/26/148/30/14

Fingerprint

Optics and Photonics
Photonic crystals
Protein Binding
Nanoparticles
Microscopy
Microscopic examination
Proteins
Biosensing Techniques
Biosensors
Imaging techniques
Computer Simulation
Adsorption
Natural frequencies
Demonstrations
Wavelength
Molecules
Computer simulation

ASJC Scopus subject areas

  • Health Informatics
  • Computer Science Applications
  • Biomedical Engineering
  • Medicine(all)

Cite this

Zhuo, Y., Tian, L., Chen, W., Yu, H., Singamaneni, S., & Cunningham, B. T. (2014). Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM). In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 (pp. 2069-2072). [6944023] (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2014.6944023

Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM). / Zhuo, Yue; Tian, Limei; Chen, Weili; Yu, Hojeong; Singamaneni, Srikanth; Cunningham, Brian T.

2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 2069-2072 6944023 (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhuo, Y, Tian, L, Chen, W, Yu, H, Singamaneni, S & Cunningham, BT 2014, Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM). in 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014., 6944023, 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014, Institute of Electrical and Electronics Engineers Inc., pp. 2069-2072, 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014, Chicago, United States, 8/26/14. https://doi.org/10.1109/EMBC.2014.6944023
Zhuo Y, Tian L, Chen W, Yu H, Singamaneni S, Cunningham BT. Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM). In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 2069-2072. 6944023. (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014). https://doi.org/10.1109/EMBC.2014.6944023
Zhuo, Yue ; Tian, Limei ; Chen, Weili ; Yu, Hojeong ; Singamaneni, Srikanth ; Cunningham, Brian T. / Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM). 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 2069-2072 (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014).
@inproceedings{155b109b3e2d44dea28ba0165402f2dd,
title = "Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM)",
abstract = "We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.",
author = "Yue Zhuo and Limei Tian and Weili Chen and Hojeong Yu and Srikanth Singamaneni and Cunningham, {Brian T.}",
year = "2014",
month = "11",
day = "2",
doi = "10.1109/EMBC.2014.6944023",
language = "English (US)",
series = "2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "2069--2072",
booktitle = "2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014",
address = "United States",

}

TY - GEN

T1 - Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM)

AU - Zhuo, Yue

AU - Tian, Limei

AU - Chen, Weili

AU - Yu, Hojeong

AU - Singamaneni, Srikanth

AU - Cunningham, Brian T.

PY - 2014/11/2

Y1 - 2014/11/2

N2 - We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.

AB - We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.

UR - http://www.scopus.com/inward/record.url?scp=84929493173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929493173&partnerID=8YFLogxK

U2 - 10.1109/EMBC.2014.6944023

DO - 10.1109/EMBC.2014.6944023

M3 - Conference contribution

C2 - 25570391

AN - SCOPUS:84929493173

T3 - 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014

SP - 2069

EP - 2072

BT - 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014

PB - Institute of Electrical and Electronics Engineers Inc.

ER -